| 固相简易荧光花环法检测人smIgG~ B细胞的研究 |
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| 引用本文： | 刘清蒙,李明强.固相简易荧光花环法检测人smIgG~ B细胞的研究[J].遵义医学院学报,1989(3). |
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| 作者姓名： | 刘清蒙 李明强 |
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| 作者单位： | 遵义医学院微生物学与免疫学教研室，遵义医学院微生物学与免疫学教研室 |
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| 摘 要： | 本文报道检测SmIgG~ B细胞的固相简易荧光花环法,30例正常人检测结果表明,该花环法较DIF法敏感(P<0.001),因不受外源性IgG和FC受体的影响,试剂制备简单、经济和稳定,标本至少可保存3个月。是一值得推广应用的检测B细胞SmIgG的有效方法。
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| 关 键 词： | 固定花环法 Cowanl 吖啶黄 SmIgG B细胞 |
The Study of Human SmIgG~ B Cell Detcted with Solid-phase Simple and Easy Fluorescence Rosette Test |
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| Liu Qingmeng Li Mingqiang.The Study of Human SmIgG~ B Cell Detcted with Solid-phase Simple and Easy Fluorescence Rosette Test[J].Acta Academiae Medicine Zunyi,1989(3). |
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| Authors: | Liu Qingmeng Li Mingqiang |
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| Abstract: | The solid-phase simple and easy fluorescence rosette test detecting SmlgG~ B lymphocytes is described. The results detected from 30 cases of normal human showed that the rosette test is more sensitive than DtF (p<0.01)It is found in the experiments that the rosette test is not affected by extrinsic cytophilic IgG and Fc receptors on some lymphocytes. The preporation of the reagent is simple, economic and stable. The sample fixed with methyl alcohol can be preserved no less three months. The rosette test is an effective method for detecting lymphocyte SmIgG. |
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| Keywords: | Solid-phase Rosette Test Cowan I Acridine Yellow SmlgG~ B Cell |
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