| DNA阵列芯片上的DNA二聚体并行热力学研究及其在SNP基因分析中的应用 |
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| 引用本文： | 李征,朱兵,高晓莲.DNA阵列芯片上的DNA二聚体并行热力学研究及其在SNP基因分析中的应用[J].华西药学杂志,2005,20(5):396-399. |
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| 作者姓名： | 李征 朱兵 高晓莲 |
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| 作者单位： | 1. 成都生物制品研究所生物技术室,四川,成都,610023
2. 休斯敦大学化学系,美国,休斯敦,77204 |
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| 摘 要： | 目的用DNA阵列芯片进行SNP的探测。方法根据DNA二聚体的热力学性质,在DNA阵列芯片上测定其解链曲线,用数学的方法获得其解链温度(Tm)值,再根据Tm值进行SNP分析。结果用特殊设计的寡聚DNA阵列芯片进行并行热力学研究,测得其DNA二聚体的解链曲线,计算出Tm值,并进行了SNP分析。结论可用DNA阵列芯片成功地进行高通量的SNP基因分析。
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| 关 键 词： | DNA DNA阵列芯片 SNP分析 解链温度 热力学研究 并行 |
| 文章编号： | 1006-0103(2005)05-0396-04 |
| 收稿时间： | 2005-03 |
| 修稿时间： | 2005年3月1日 |
Parallel thermodynamic analysis of duplexes on oligo DNA microarray and its important usage in SNP analysis |
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| LI Zheng,ZHU Bing,GAO Xiao-lian.Parallel thermodynamic analysis of duplexes on oligo DNA microarray and its important usage in SNP analysis[J].West China Journal of Pharmaceutical Sciences,2005,20(5):396-399. |
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| Authors: | LI Zheng ZHU Bing GAO Xiao-lian |
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| Institution: | 1. Department of Biotechniques, Chengdu Institute of Biological Products, Chengdu 610023, China ;2. Department of Chemistry, University of Houston, Houston 77204, USA |
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| Abstract: | OBJECTIVE To analyze SNP by oligo DNA microarray.METHODS Melting curves were determined in the DNA microarray,in terms of the thermodynamic traits of DNA duplexes,and the Tm values was calculated for SNP analysis.RESULTS With the special DNA microarray,thermodynamic analysis has done to get the melting curve of the DNA duplexes as well as their Tm values for SNP analysis.CONCLUSION It is likely to do high-throughput SNP analysis by DNA microarray. |
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| Keywords: | DNA DNA microarray SNP analysis Tm Thermodynamic analysis Parallel |
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