RP-HPLC测定人血浆中卡托普利浓度及其药动学

 目的建立测定人血浆中卡托普利的反相高效液相色谱法,研究其在人体内的药动学规律及特点。方法采用美国Waters公司高效液相色谱仪。色谱柱Hypersil柱(4.6mm×250mm,5μm);流动相为乙腈-水-冰醋酸(45∶55∶0.2),流速1.0mL·min^-1;检测波长:257nm。结果卡托普利血药浓度线性范围为6.25～800μg·L^-1,最低检出浓度为6.25μg·L^-1;方法回收率为105.8%;日内、日间RSD分别为4.40%～7.88%和2.87%～9.34%。结论本法灵敏、准确,可用于卡托普利临床药动学研究。

Determination of Captopril in Human Plasma by RP-HPLC

OBJECTIVE To establish a reversed phase high performance liquid chromatographic method for the determination of captopril in human plasma and to investigate the pharmacokinetics of captopril in Chinese healthy volunteers.METHODS The drug was extracted from human plasma with ethyl acetate-benzene(1∶1).The plasma concentrations of captopril were separated on a HYPERSIL C₁₈ column(4.6 mm×250 mm,5 μm)by Waters 510 HPLC.The mobile phase was a mixture of acetonitrile-1%acetic acid(45∶55).The detection wavelength was 257 nm.RESULTS A good linearity was obtained over the range of 6.25 to 800 μg·L^-1 in human plasma.The limit of detection was 6.25 μg·L^-1.The recoverys of method were from 101.00% to 108.88%.The precisions of intra-day and inter-day were in the rangs of 4.40%～7.88% and 2.87%～9.34%,respectively.CONCLUSION The method is sensitive,accurate,reliable,and can be used in clinical research of captopril.