茶多酚对大鼠肾缺血再灌注损伤保护作用实验研究

目的探讨茶多酚（Tea polyphenols TP）预处理对大鼠肾缺血再灌注损伤的保护作用及其机制。方法建立大鼠肾缺血再灌注损伤（renal ischemia reperfusion injury RIRI）模型。选用健康SD大鼠42只,随机分为3组：假手术组、单纯肾缺血再灌注损伤组,茶多酚预处理组。于肾缺血再灌注（renal ischemia reperfusion RIR）开始时刻、RIR后2h、RIR后24h分别检测血清肌酐（SCr）,血清超氧化物岐化酶（SOD）、血清丙二醛（MDA）、肾组织SOD、肾组织MDA及观察肾组织的病理变化。结果茶多酚预处理组与假手术组相比较,RIRI组SCr水平升高（P〈0.05）,肾组织及血清中的SOD降低及MDA水平增加（P〈0.05）。而茶多酚预处理组SCr、血清MDA和肾组织MDA均比单纯RIRI组低（P〈0.05）,血清SOD水平及肾组织SOD水平升高（P〈0.05）。结论在肾脏缺血再灌注损伤过程中,茶多酚能起到对肾脏的保护作用。

Experimental Study about the Protective Effect of Tea Polyphenols on Acute Renal Ischemia Reperfusion

Objective To investigate the protective effects and it＇s mechanism of tea polyphenols on acute renal ischemia reperfusion injury（RIRI） in rats models.Methods The model of rat＇s RIRI was produced.42 SD rats were randomly divided into 3 groups：Shamoperated group（group S）,renal ischemia reperfusion groups（group RIRI） and tea polyphenols precondiontioning groups（group TP）.The levels of SCr,Serum Super oxide Dimutase（SOD）,mlondialdehyde（MDA）,renal tissue＇s SOD,renal tissue＇s MDA in each group were respectively detected RIR for 0 minutes and after RIR for after 2 hours and after RIR 24hours,Pathological changes were also examined by light microscope.Results Tea polyphenols preconditioning decreased the levels of SCr significantly（P〈0.05）.There were statistic difference of the serum levels of SOD,MDA and renal tissue＇s SOD,MDA in tea polyphenols preconditioning group and RIR group（P〈0.05）.Conclusion Tea polyphenols preconditioning group to kidney could protect the kidney against renal ischemia reperfusion injury.