| IFRD1基因在大鼠胚胎心脏发育中的表达 |
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| 引用本文： | 孙凤荣,龚杰,钱玲梅,孔祥清,盛燕辉,杨荣,曹克将.IFRD1基因在大鼠胚胎心脏发育中的表达[J].实用儿科临床杂志,2006,21(20):1402-1404. |
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| 作者姓名： | 孙凤荣 龚杰 钱玲梅 孔祥清 盛燕辉 杨荣 曹克将 |
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| 作者单位： | 南京医科大学第一附属医院,心脏科,南京,210029 |
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| 基金项目： | 国家自然科学基金;江苏省自然科学基金 |
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| 摘 要： | 目的检测大鼠胚胎心脏发育过程中IFRD1基因mRNA及蛋白的表达。方法取大鼠胚胎12、15、19 d心脏,应用RT-PCR、Western-blot、免疫组织化学技术检测胚胎大鼠心脏IFRD1基因mRNA及蛋白表达。结果在大鼠胚胎12、15、19 d心脏中,IFRD1基因mRNA及蛋白均有表达,且呈逐渐下调趋势;其蛋白主要表达于心房、心室、肌小梁等处心肌组织,而在心内膜垫、瓣膜、流出道、大血管内层等处未见表达。结论IFRD1基因的表达随大鼠胚胎心脏的生长发育逐渐下调,且特异表达于大鼠胚胎心脏心房、心室、肌小梁等处心肌组织,可能与心脏发育密切相关。
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| 关 键 词： | IFRD1基因 大鼠 胚胎 心脏 发育 |
| 文章编号： | 1003-515X(2006)20-1402-03 |
| 收稿时间： | 2006-08-01 |
| 修稿时间： | 2006年8月1日 |
Expression of Interferon- Related Developmental Requlator 1 Gene during Development of Embryos Hearts in Rat |
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| SUN Feng-rong,GONG Jie,QIAN Ling-mei,KONG Xiang-qing,SHENG Yan-hui,YANG Rong,CAO Ke-jiang.Expression of Interferon- Related Developmental Requlator 1 Gene during Development of Embryos Hearts in Rat[J].Journal of Applied Clinical Pediatrics,2006,21(20):1402-1404. |
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| Authors: | SUN Feng-rong GONG Jie QIAN Ling-mei KONG Xiang-qing SHENG Yan-hui YANG Rong CAO Ke-jiang |
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| Institution: | Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China |
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| Abstract: | Objective To investigate the expression of interferon-related developmental regulator 1(IFRD1) gene during development of embryos hearts in rat.Method Rat hearts in embryonic 12~th,15~th,19~th day were included and the expression of IFRD1 gene was detected by RT-PCR,Western-Blot and immunohistochemistry.Results IFRD1 gene was expressed in rat hearts in 12~th,15~th,19~th day.The expression decreased during development of rat hearts.IFRD1 gene was mainly expressed in the atria,ventricles and trabecular muscles,while not in atrial-ventricular valves,outflow tracks and large arteries.Conclusion IFRD1 gene is down-regulated during development of rat hearts and mainly expression in the atria,ventricles and trabecular muscles. |
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| Keywords: | interferon-related developmental regulator 1 gene rattus embryos heart developing |
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