胰岛素对百草枯诱导损伤PC12细胞的保护机制的研究

目的 探讨胰岛素对百草枯(PQ)诱导损伤的多巴胺能神经元PC12细胞保护作用的可能机制.方法 在前期实验证明胰岛素对PQ诱导损伤的PC12细胞具有保护作用的基础上,应用免疫沉淀Western印迹分析技术检测空白对照PC12细胞组、PQ干预PC12细胞组(PQ组)、胰岛素干预PC12细胞组(胰岛素组)和PQ与胰岛素共同干预PC12细胞组(胰岛素+PQ组)INRTyr~(1162/1163)和AktSer~(473)磷酸化蛋白的表达情况.结果 免疫沉淀Western印迹分析技术显示:胰岛素组和胰岛素+PQ组通道上出现了表示INRTyr~(1162/1163)磷酸化蛋白表达的棕色带,没有胰岛素干预的通道均未出现棕色带;INR Tyr~(1162/1163)磷酸化蛋白表达量化统计分析显示胰岛素组INRTyr~(1162/1163)磷酸化程度高于胰岛素+PQ组,但差异无统计学意义(P>0.05);而AktSer~(473)磷酸化蛋白表达量化统计分析显示胰岛素组AktSer~(473)磷酸化程度明显高于胰岛素+PQ组,差异有统计学意义(P<0.05).结论 INR Tyr~(1162/1163)磷酸化增加和胰岛素受体后信号传导通路AktSer~(473)磷酸化增加可能是胰岛素保护PQ诱导损伤PC12细胞的机制之一.

Protective mechanism of insulin on paraquat-induced PC12 cells

Objective To investigate the protective mechanism of insulin on paraquat-induced PC12 cells. Methods Based on the Part (insulin can protect the paraquat-induced PC12 cells), immunoprecipitation and Western blotting were employed to observe the protein expressions of INR Tyr~(1162/1163) and AktSer~(473) in the group of normal PC12 cells, group of insulin interfered PC12 cells, group of PQ-induced PC12 cells and group of PQ combined with insulin interfered PC12 cells, respectively. Results Group of insulin interfered PCI2 cells and group of PQ combined with insulin interfered PC12 cells showed expression of phosphorylation protein INR Tyr~(1162/1163), while the other groups without insulin intervention did not show the expression of that. The expression of phosphorylation protein INR Tyr~(1162/1163) in the group of insulin interfered PC12 cells was higher than that in the group of PQ combined with insulin interfered PC12 cells, but statistical analysis showed no significant difference between the 2 groups (P>0.05). Meanwhile, the expression of phosphorylation protein AktSer~(473) in the group of insulin interfered PC 12 cells was obviously higher than that in the group of PQ combined with insulin interfered PC12 cells (P<0.05). Conclusion The increased expression of phosphorylation protein INR Tyr~(1162/1163) and AktSer~(473) might be one of the clues in proving that insulin may have the protective effect on PC12 cells.