Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction |
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| Authors: | B R Madewell P H Gumerlock K A Saunders F J Meyers S A Kraegel E S Kawasaki |
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| Affiliation: | Department of Veterinary Surgery, University of California School of Veterinary Medicine, Davis 95616. |
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| Abstract: | Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR). |
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