四逆汤抗心肌缺血-再灌注线粒体氧化损伤作用及机制的研究

目的:阐明四逆汤抗心肌缺血再灌注的线粒体氧化损伤作用及机制。方法:将昆明种小鼠分为对照组、缺血再灌注模型组、四逆汤治疗组,采用腹腔注射Pit(垂体后叶素)制造小鼠心肌缺血再灌注模型。结果:四逆汤可以明显提高心肌组织及线粒体内SOD活性(P<0.01),明显降低心肌组织及线粒体内MDA含量(P<0.01),减少心肌组织内乳酸含量(P<0.05),四逆汤还可以明显减轻心肌缺血-再灌注损伤时线粒体肿胀程度(P<0.01),明显增强线粒体MnSODmRNA的转录(P<0.01)结论:四逆汤可以明显减轻心肌缺血-再灌注过程中的线粒体氧化损伤,其机制为增强线粒体MnSODmRNA的表达。

Study on Activity and Mechanism of Sini Decoction Anti-mitochondrial Oxidation Injury Caused by Myocardial lschemia/Reperfusion

Objective:To study the activity and mechanism of Sini Decoction(SND) Anti-mitochondrial Oxidation Injury caused by Myocardial Ischemia/Reperfusion.Methods:Kun ming mice were randomly divided into three groups:Control group,Ischemia/Reperfusion(I/R) group and SND-treated group.At the end of experiment,hearts of mice were taken out for further detection.Activity of myocardium and mitochondrial SOD,content of myocardium and mitochondrial MDA,swelling of mitochondria,Lactic Acid content of myocardium and MnSODmRNA expression were observed.Results:SND could increase the activity of myocardium and mitochondrial SOD(P<0.01),decrease the content of myocardium and mitochondrial MDA(P<0.01),decrease the Lactic Acid content of myocardium,lighted the swelling of mitochondria(P<0.01)and change the expression of MnSODmRNA(P<0.01).Conclusion:Sini decoction can anti-mitochondrial oxidation injury caused by Myocardial Ischemia/Reperfusion,its mechanism may be relate to increasing the MnSODmRNA expression.