| 表达HBVX基因的小鼠模型的建立及对肝细胞凋亡因子的影响 |
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| 引用本文： | 郭晨,李丹,林纳,黄月红,陈治新,王小众. 表达HBVX基因的小鼠模型的建立及对肝细胞凋亡因子的影响[J]. 世界华人消化杂志, 2011, 0(12) |
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| 作者姓名： | 郭晨 李丹 林纳 黄月红 陈治新 王小众 |
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| 作者单位： | 福建医科大学附属协和医院消化内科; |
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| 摘 要： | 目的:探讨乙型肝炎病毒X(HBVX)基因及其产物对肝细胞凋亡相关基因表达的影响.方法:将实验用SPF级成年♂KM小鼠分为实验组、空质粒对照组、生理盐水对照组.实验组以构建好的PCDNA3.1-HBVX质粒,空质粒对照组以PCDNA3.1质粒,生理盐水对照组以生理盐水,通过尾静脉高压注入动物体内,在第48小时处死小鼠后,取肝组织,以RT-PCR,凝胶回收测序及Westernblot检测HBVX表达,以RT-PCR半定量检测小鼠肝组织内bax、c-myc及bcl-2的表达.结果:实验组RT-PCR显示465bp处有清楚条带,肝组织内有HBVXmRNA的存在,两对照组无HBVXmRNA存在,Western blot检测实验组有HBVX蛋白的表达;两对照组则无HBVX蛋白表达.通过对小鼠肝组织凋亡相关因子的半定量RT-PCR,相对于空质粒对照组和生理盐水对照组,转染HBVX基因的小鼠肝组织bax、c-myc及bcl-2的mRNA相对表达量明显增高,差异有显著性意义(bax:1.3127±0.0900vs1.0023±0.1670,0.9094±0.1081;c-myc:1.6294±0.0672vs1.2869±0....
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| 关 键 词： | 乙型肝炎病毒X基因 肝细胞 凋亡 小鼠模型 |
Establishment of a mouse model harboring the hepatitis B virus X gene and analysis of expression of apoptosis factors in liver cells of this mouse line |
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| Chen Guo,Dan Li,Na Lin,Yue-Hong Huang,Zhi-Xin Chen,Xiao-Zhong Wang. Establishment of a mouse model harboring the hepatitis B virus X gene and analysis of expression of apoptosis factors in liver cells of this mouse line[J]. World Chinese Journal of Digestology, 2011, 0(12) |
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| Authors: | Chen Guo Dan Li Na Lin Yue-Hong Huang Zhi-Xin Chen Xiao-Zhong Wang |
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| Affiliation: | Chen Guo,Dan Li,Na Lin,Yue-Hong Huang,Zhi-Xin Chen,Xiao-Zhong Wang,Department of Gastroenterology,Union Hospital,Fujian Medical University,Fuzhou 350001,Fujian Province,China |
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| Abstract: | AIM: To investigate the impact of the hepatitis B virus X gene (HBVX) on the expression of apoptosis factors in liver cells.METHODS: The eukaryotic expression vector PCDNA3.1-HBVX was injected into the caudal vein of mice to establish a mouse model expressing the HBVX gene. Mice injected with empty PCDNA3.1 vector or normal saline were used as controls. RT-PCR and Western blot were performed to detect the expression of HBVX in liver tissue 48 h after injection. Semi-quantitative RT-PCR was used to evaluate ... |
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| Keywords: | Hepatitis B virus X gene Liver cells Apoptosis Mouse model |
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