| 乙型肝炎病毒X蛋白对人肝L-02细胞中COX-2表达的影响 |
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| 引用本文： | 靳梦杰,刘慧涛,赵国强. 乙型肝炎病毒X蛋白对人肝L-02细胞中COX-2表达的影响[J]. 世界华人消化杂志, 2011, 0(18) |
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| 作者姓名： | 靳梦杰 刘慧涛 赵国强 |
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| 作者单位： | 郑州大学基础医学院;武警河南总队郑州市支队卫生队;南阳市油田总医院; |
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| 摘 要： | 目的:研究在人肝细胞L-02中乙型肝炎病毒X蛋白(HBx)对环氧化酶2(COX-2)表达的影响.方法:构建HBx基因表达载体pIRES2-AcGFP-HBx,转染入人肝细胞L-02中,RT-PCR和Westernblot检测HBx对COX-2表达的影响;通过细胞生长曲线和细胞周期变化,检测HBx蛋白对细胞分裂增殖的作用.同时将含有COX-2启动子核心片段的荧光素酶报告载体pGL3-COX-2转染入上述转染细胞,检测细胞荧光素酶荧光值以观察HBx蛋白对COX-2启动子活性的影响.结果:RT-PCR结果显示仅在HBx基因转染组细胞中有HBxmRNA的表达,且该组细胞中COX-2mRNA相对表达量显著高于空载体对照组和空白对照组(0.76±0.12vs0.28±0.04,0.25±0.03,均P<0.01);Westernblot结果显示,仅HBx基因转染组可见HBx蛋白有明显印迹条带,且该组细胞中COX-2蛋白免疫印迹较两对照组深;细胞生长曲线测定表明HBx基因转染组细胞在第3、4、5天的生长较两对照组显著加快(P<0.05);细胞周期测定显示与两对照组相比,HBx基因转染组G0-G1期比例显著降低,S期和G2-...
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| 关 键 词： | 乙型肝炎病毒 乙型肝炎病毒X蛋白 环氧化酶2 肝癌 |
Hepatitis B virus X protein enhances COX-2 expression in human liver cell line L-02 |
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| Meng-Jie Jin,Guo-Qiang Zhao,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou ,Henan Province,China Meng-Jie Jin,Medical Team,Zhengzhou Regiment of China Armed Police Force,Zhengzhou ,China Hui-Tao Liu,Nanyang Oilfield General Hospital,Nanyang ,China. Hepatitis B virus X protein enhances COX-2 expression in human liver cell line L-02[J]. World Chinese Journal of Digestology, 2011, 0(18) |
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| Authors: | Meng-Jie Jin Guo-Qiang Zhao College of Basic Medical Sciences Zhengzhou University Zhengzhou Henan Province China Meng-Jie Jin Medical Team Zhengzhou Regiment of China Armed Police Force Zhengzhou China Hui-Tao Liu Nanyang Oilfield General Hospital Nanyang China |
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| Affiliation: | Meng-Jie Jin,Guo-Qiang Zhao,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001,Henan Province,China Meng-Jie Jin,Medical Team,Zhengzhou Regiment of China Armed Police Force,Zhengzhou 450006,China Hui-Tao Liu,Nanyang Oilfield General Hospital,Nanyang 473132,China |
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| Abstract: | AIM:To investigate the effect of hepatitis B virus X protein(HBx) on COX-2 expression in human liver cell line L-02.METHODS:HBx expression vector pIRES2AcGFP-HBx was constructed and transfected into L-02 cells.The expression of COX-2 mRNA and protein was detected by RT-PCR and Western blot,respectively.The effect of HBx protein on cell division and proliferation was evaluated by plotting cell growth curve and analyzing cell cycle.Moreover,pGL3-COX-2 plasmid,in which the COX-2 promoter has been linked to the... |
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| Keywords: | Hepatitis B virus Hepatitis B virus X protein Cyclooxygenase-2 Liver cancer |
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