| siRNA沉默USP22基因对胃癌细胞增殖的抑制作用 |
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| 引用本文： | 邓美洲,陶凯雄,王国斌,刘兴华.siRNA沉默USP22基因对胃癌细胞增殖的抑制作用[J].世界华人消化杂志,2011(19). |
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| 作者姓名： | 邓美洲 陶凯雄 王国斌 刘兴华 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院腔镜外科; |
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| 摘 要： | 目的:探讨小干扰RNA(siRNA)沉默泛素特异性肽酶22(ubiquitin specific peptidase22,USP22)对胃癌细胞增殖的影响.方法:针对USP22基因设计3条siRNA及阴性siRNA,用脂质体Lipofectamine 2000转染胃癌AGS细胞,通过实时定量PCR和Western blot检测转染后AGS细胞USP22基因中mRNA和蛋白表达水平的变化情况,流式细胞术检测细胞周期分布变化情况,CCK8法检测细胞增殖率及抑制率.结果:转染48h后,3条siRNA均能显著抑制USP22 mRNA和蛋白的表达.其中,以转染USP22 siRNA3后效果最明显,mRNA和蛋白表达分别下降80.47%±2.99%和79.40%±3.58%.细胞增殖明显受到抑制,USP22 siR-NA3组细胞增殖抑制率为27.33%±3.49%.细胞周期中G0/G1期细胞增多,S期细胞减少.结论:采用RNA干扰技术能够有效地沉默USP22基因的表达,并显著抑制胃癌细胞的增殖.
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| 关 键 词： | 泛素特异性肽酶22 小干扰RNA 胃癌 AGS细胞 细胞增殖 |
SiRNA-mediated silencing of the USP22 gene inhibits cell proliferation in human gastric cancer cell line AGS |
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| Mei-Zhou Deng,Kai-Xiong Tao,Guo-Bin Wang,Xing-Hua Liu.SiRNA-mediated silencing of the USP22 gene inhibits cell proliferation in human gastric cancer cell line AGS[J].World Chinese Journal of Digestology,2011(19). |
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| Authors: | Mei-Zhou Deng Kai-Xiong Tao Guo-Bin Wang Xing-Hua Liu |
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| Institution: | Mei-Zhou Deng,Kai-Xiong Tao,Guo-Bin Wang,Xing-Hua Liu,Department of Laparoscopic Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China |
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| Abstract: | AIM:To evaluate the impact of silencing of the USP22 gene by small interfering RNA(siRNA) on the proliferation of human gastric cancer AGS cells. METHODS:Three USP22-specific siRNAs and a negative siRNA were designed and transfected into AGS cells using Lipofectamine 2000.Quantitative real-time PCR(qRT-PCR)and Western blot were utilized to detect the expression levels of USP22 mRNA and protein,respectively.Cell proliferation was measured using Cell Counting Kit-8(CCK-8).The distribution of cell cycle was de... |
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| Keywords: | Ubiquitin-specific peptidase 22 Small interfering RNA Gastric cancer AGS cells Cell proliferation |
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