| siRNA沉默Bmi-1表达对胰腺癌PANC-1细胞增殖的抑制作用 |
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| 引用本文： | 冷政伟,殷涛,夏清华,张万里,王国斌,陶凯雄. siRNA沉默Bmi-1表达对胰腺癌PANC-1细胞增殖的抑制作用[J]. 世界华人消化杂志, 2011, 0(13): 1342-1346 |
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| 作者姓名： | 冷政伟 殷涛 夏清华 张万里 王国斌 陶凯雄 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院普外科; |
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| 摘 要： | 目的:探讨运用RNAi技术沉默Bmi-1基因对人胰腺癌细胞恶性行为的影响,为胰腺癌基因治疗提供新的靶点和思路.方法:构建反义Bmi-1真核表达载体转染人胰腺癌PANC-1细胞,运用荧光显微镜下观察、MTT、Western blot、流式细胞术检测转染效果及细胞周期、凋亡变化.结果:成功构建反义Bmi-1真核表达载体并且...
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| 关 键 词： | 胰腺癌 B细胞特异性莫洛尼氏白血病毒插入位点1 小干扰RNA 流式细胞术 |
SiRNA-mediated downregulation of Bmi-1 expression inhibits proliferation of human pancreatic cancer PANC-1 cells |
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| Zheng-Wei Leng,Tao Yin,Qing-Hua Xia,Wan-Li Zhang,Guo-Bin Wang,Kai-Xiong Tao. SiRNA-mediated downregulation of Bmi-1 expression inhibits proliferation of human pancreatic cancer PANC-1 cells[J]. World Chinese Journal of Digestology, 2011, 0(13): 1342-1346 |
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| Authors: | Zheng-Wei Leng Tao Yin Qing-Hua Xia Wan-Li Zhang Guo-Bin Wang Kai-Xiong Tao |
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| Affiliation: | Zheng-Wei Leng,Tao Yin,Qing-Hua Xia,Wan-Li Zhang,Guo-Bin Wang,Kai-Xiong Tao,Department of General Surgery,Wuhan Union Hospital,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China |
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| Abstract: | AIM:To investigate the impact of knockdown of the B-cell specific Moloney leukemia virus insertion site 1(Bmi-1)gene on the biological behavior of human pancreatic cancer cells.METHODS:An expression vector carrying a small interference RNA(siRNA)targeting the Bmi-1 gene was constructed and transfected into human pancreatic cancer PANC-1 cells.After transfection,Bmi-1 protein expression was detected by Western blot;cell proliferation was evaluated by MTT assay;and cell cycle progression and apoptosis were ex... |
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| Keywords: | Pancreatic cancer B-cell specific Moloney leukemia virus insertion site 1 Small interference RNA Flow cytometry |
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