| 溶癌水泡性口炎病毒体外诱导人肝癌细胞HepG2凋亡的作用机制 |
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| 引用本文： | 连海,汤晶,孙玉成,张爽.溶癌水泡性口炎病毒体外诱导人肝癌细胞HepG2凋亡的作用机制[J].世界华人消化杂志,2011(7):667-673. |
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| 作者姓名： | 连海 汤晶 孙玉成 张爽 |
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| 作者单位： | 军事医学科学院十一所基础研究室; |
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| 基金项目： | 国家自然科学基金资助项目,No. 31001070; 吉林省科技发展计划基金资助项目,No. 20100146~~ |
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| 摘 要： | 目的:研究一株实验室减毒水泡性口炎病毒(VSV)对HepG2细胞的凋亡诱导作用,并探讨其可能的作用机制.方法:首先将水泡性口炎病毒以1.0感染复数(MOI)的接种密度感染HepG2细胞,同时设接种相同体积培养液的Mock感染组,在不同时间点收集细胞,MTT法检测细胞增殖活性;AO/EB结合Hoechst/PI染色观察细...
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| 关 键 词： | 水泡性口炎病毒 肝细胞癌 凋亡 HepG2细胞 |
Mechanisms underlying vesicular stomatitis virusinduced apoptosis of HepG2 cells in vitro |
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| Hai Lian,Jing Tang,Yu-Cheng Sun,Shuang Zhang.Mechanisms underlying vesicular stomatitis virusinduced apoptosis of HepG2 cells in vitro[J].World Chinese Journal of Digestology,2011(7):667-673. |
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| Authors: | Hai Lian Jing Tang Yu-Cheng Sun Shuang Zhang |
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| Institution: | Hai Lian,Jing Tang,Yu-Cheng Sun,Shuang Zhang,Department of Basic Medical Sciences,the Eleventh Institute of Academy of Military Medical Sciences,Changchun 130122,Jilin Province,China |
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| Abstract: | AIM:To investigate the apoptosis-inducing effect of a laboratory-attenuated vesicular stomatitis virus(VSV) strain on HepG2 cells and to explore the underlying mechanisms.METHODS:After HepG2 cells were infected with VSV at a multiplicity of infection(MOI) of 1.0,cell viability was determined by MTT assay morphological assessment of apoptosis was performed by acridine orange(AO)/ethidium bromide(EB) and Hoechst/PI staining;apoptotic cells were quantified by annexin V/PI doublestaining and cell cycle analysis... |
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| Keywords: | Vesicular stomatitis virus Hepatocellular carcinoma Apoptosis HepG2 cells |
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