| 塞莱昔布对幽门螺杆菌蛋白质组的影响 |
| |
| 引用本文： | 高培培,王蔚虹,王静,李江,董欣红. 塞莱昔布对幽门螺杆菌蛋白质组的影响[J]. 世界华人消化杂志, 2011, 0(17) |
| |
| 作者姓名： | 高培培 王蔚虹 王静 李江 董欣红 |
| |
| 作者单位： | 北京大学第一医院消化内科; |
| |
| 基金项目： | 国家自然科学基金资助项目,No.30770981~~ |
| |
| 摘 要： | 目的:探讨选择性COX-2抑制剂塞莱昔布对幽门螺杆菌(H.pylori)蛋白质组的影响.方法:双向凝胶电泳技术(2-DE)分离塞莱昔布处理前后的H.pylori标准株26695全菌蛋白,银染法凝胶染色,图像扫描分析软件识别差异蛋白,基质辅助激光解吸电离飞行时间质谱(MALD-TOF-MS)、串联质谱(MALDI-TOF-MS/MS)分析差异蛋白;SYBRGreenⅠ实时定量PCR测定差异蛋白基因的表达变化.结果:塞莱昔布处理后,H.pylori标准株26695发现17个蛋白质斑点表达有差异.7个蛋白质斑点得到阳性鉴定,分别归为H.pylori的3种蛋白质:热休克蛋白60(HSP60),即groEL,延伸因子(EF-TU),谷氨酰转肽酶(GGT).与DMSO溶媒对照相比,塞莱昔布处理后的H.pyloriHSP60(groEL)、EF-TU、GGT表达下调.实时定量PCR测定显示,编码H.pyloriEF-TU、GGT基因的mRNA水平降低(0.07±0.06vs1.01±0.16;0.31±0.13vs0.98±0.01,均P<0.05),而编码H.pyloriHSP60(groEL)基因的mRNA水平增加(1...
|
| 关 键 词： | 幽门螺杆菌 塞莱昔布 蛋白质组 |
Proteomic profiling of Helicobacter pylori treated with celecoxib |
| |
| Pei-Pei Gao,Wei-Hong Wang,Jing Wang,Jiang Li,Xin-Hong Dong. Proteomic profiling of Helicobacter pylori treated with celecoxib[J]. World Chinese Journal of Digestology, 2011, 0(17) |
| |
| Authors: | Pei-Pei Gao Wei-Hong Wang Jing Wang Jiang Li Xin-Hong Dong |
| |
| Affiliation: | Pei-Pei Gao,Wei-Hong Wang,Jing Wang,Jiang Li,Xin-Hong Dong,Department of Gastroenterology,Peking University First Hospital,Beijing l00034,China |
| |
| Abstract: | AIM: To perform a proteomic investigation of the effect of celecoxib on Helicobacter pylori (H.pylori). METHODS: Total proteins of untreated and celecoxib-treated H.pylori 26695 were extracted and separated by 2-dimensionals polyacrylamide gel electrophoresis (2-DE). Differential protein expression was detected using computer-assisted image analysis. Differential proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and matrix-assisted laser ... |
| |
| Keywords: | Helicobacter pylori Celecoxib Proteomics |
| 本文献已被 CNKI 等数据库收录！ |
|
