| 白花丹醌对人肝星状细胞凋亡及相关蛋白表达的影响 |
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| 引用本文： | 韦燕飞,钟振国,黄仁彬,彭岳,谢海源,段雪琳,赵铁建.白花丹醌对人肝星状细胞凋亡及相关蛋白表达的影响[J].世界华人消化杂志,2011(4). |
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| 作者姓名： | 韦燕飞 钟振国 黄仁彬 彭岳 谢海源 段雪琳 赵铁建 |
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| 作者单位： | 广西医科大学药理实验室;广西中医学院基础医学院生理教研室;广西中医学院新药开发中心; |
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| 基金项目： | 国家自然科学基金资助项目,No.30760321; 广西科学研究与技术开发基金资助项目,No.0992003-1~~ |
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| 摘 要： | 目的:研究白花丹醌对瘦素(Leptin)刺激体外培养人肝星状细胞株HSC-LX2凋亡及相关蛋白表达的影响,以探讨其抗肝纤维化作用的机制.方法:体外培养HSC-LX2,将其分为以下几组:空白对照组、Leptin对照组、秋水仙碱组、2、8、16μmol/L白花丹醌组.瘦素刺激24h,药物与细胞共孵育24h后,应用流式细胞仪Annexin/PI双染方法检测细胞凋亡,透射电镜观察HSC-LX2凋亡形态学变化,免疫组织化学法检测HSC-LX2凋亡基因p53、Bax、Bcl-2的蛋白表达.结果:细胞流式术结果显示,白花丹醌作用HSC-LX224h,HSC凋亡率明显增加,白花丹醌8、16μmol/L组和秋水仙碱组HSC凋亡率均显著高于空白对照组和Leptin对照组(5.21±0.41,8.10±0.63,10.1±1.08vs1.40±0.13,2.85±0.21,均P<0.01).透射电镜结果,空白对照组细胞形态清晰,细胞膜、核膜完整,胞质内细胞器清晰,染色质均匀,细胞表面微绒毛;药物组可见少量坏死细胞和许多不同程度的凋亡细胞,体积变小,核膜消失,胞质浓缩,胞质内出现大量空泡,细胞核染色质固缩,可见核碎裂,细胞表面微绒毛...
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| 关 键 词： | 白花丹醌 肝星状细胞 细胞凋亡 P53 Bax Bcl-2 |
Effects of plumbagin on apoptosis and expression of apoptosis-related proteins in human hepatic stellate cells |
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| Yan-Fei Wei,Ren-Bin Huang,Pharmacological Laboratory of Guangxi Medical University,Nanning ,Guangxi Zhuang Autonomous Region,China Yan-Fei Wei,Yue Peng,Hai-Yuan Xie,Xue-Lin Duan,Tie-Jian Zhao.Effects of plumbagin on apoptosis and expression of apoptosis-related proteins in human hepatic stellate cells[J].World Chinese Journal of Digestology,2011(4). |
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| Authors: | Yan-Fei Wei Ren-Bin Huang Pharmacological Laboratory of Guangxi Medical University Nanning Guangxi Zhuang Autonomous Region China Yan-Fei Wei Yue Peng Hai-Yuan Xie Xue-Lin Duan Tie-Jian Zhao |
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| Institution: | Yan-Fei Wei,Ren-Bin Huang,Pharmacological Laboratory of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China Yan-Fei Wei,Yue Peng,Hai-Yuan Xie,Xue-Lin Duan,Tie-Jian Zhao,Department of Physiology,Guangxi Traditional Chinese Medical University,Nanning 530001,China Zhen-Guo Zhong,Center for New Drug Research,China |
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| Abstract: | AIM:To investigate the effects of plumbagin on leptin-induced apoptosis and expression of apoptosis-related protein in human hepatic satellite cells(HSC-LX2) and to explore the anti-fibrotic mechanism of plumbagin.METHODS:After HSC-LX2 cells were cultured in vitro,stimulated with leptin for 24 h,and treated with different concentrations of plumbagin for 24 h,cell apoptosis was detected by flow cytometry;cell ultrastructure was observed by transmission electron microscopy;and the protein expression of P53,Ba... |
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| Keywords: | Plumbagin Hepatic stellate cells Apoptosis P53 Bax Bcl-2 |
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