| 自噬相关基因LC3和Beclin1在大鼠急性坏死性胰腺炎中的表达和意义 |
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| 引用本文： | 杨淑丽,陈卫昌,孙楹,陈冯梅. 自噬相关基因LC3和Beclin1在大鼠急性坏死性胰腺炎中的表达和意义[J]. 世界华人消化杂志, 2011, 0(12) |
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| 作者姓名： | 杨淑丽 陈卫昌 孙楹 陈冯梅 |
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| 作者单位： | 苏州大学附属第一医院消化内科;苏州卫生职业技术学院; |
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| 基金项目： | 江苏省普通高校研究生科研创新计划基金资助项目,No.CX10B-0472~~ |
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| 摘 要： | 目的:研究大鼠急性坏死性胰腺炎(ANP)胰腺组织LC3、Beclin1的表达,并探讨其在ANP中的作用及意义.方法:36只SD大鼠随机分为假手术(SO)组和ANP组,ANP组采用胰胆管逆行注射5%牛磺胆酸钠诱导制作大鼠ANP模型,SO组开腹后翻动胰腺后关腹,各组再分3、6和12h3个不同时间点.观察各组大鼠血清炎症因子及胰腺的病理改变,运用免疫组织化学法检测各组大鼠在不同时间点胰腺组织LC3和Beclin1的变化.结果:ANP组血浆淀粉酶(U/L)、TNF-α(μg/L)较SO组明显增加(血浆淀粉酶:3h:4936±1207vs1447±355;6h:5464±768vs1513±333;12h:6139±710vs1539±231;TNF-α:3h:111.24±21.86vs56.14±7.69;6h:107.55±33.05vs57.13±11.30;12h:108.24±24.83vs58.60±9.54,均P<0.05),且ANP组大鼠胰腺病理改变随着时间延长加重.正常大鼠胰腺腺泡细胞LC3、Beclin1低表达,大鼠ANP3h后胰腺细胞LC3、Beclin1表达开始增多,在6和12h呈强表达.结论...
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| 关 键 词： | 自噬 急性坏死性胰腺炎 微管相关蛋白1轻链3 Beclin1 |
Signifi cance of expression of autophagy-related proteins LC3 and Beclin-1 in acute necrotizing pancreatitis in rats |
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| Shu-Li Yang,Wei-Chang Chen,Ying Sun. Signifi cance of expression of autophagy-related proteins LC3 and Beclin-1 in acute necrotizing pancreatitis in rats[J]. World Chinese Journal of Digestology, 2011, 0(12) |
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| Authors: | Shu-Li Yang Wei-Chang Chen Ying Sun |
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| Affiliation: | Shu-Li Yang,Wei-Chang Chen,Ying Sun,Department of Gastroenterology,the First Affi liated Hospital of Soochow University,Suzhou 215006,Jiangsu Province,ChinaShu-Li Yang,Feng-Mei Chen,Suzhou Health College,China |
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| Abstract: | AIM: To examine the expression of microtubuleassociated protein 1 light chain 3 (LC3) and Be-clin-1 in acute necrotizing pancreatitis (ANP) in rats and to analyze their signif icance.METHODS: Thirty-six Sprague-Dawley rats were randomized into sham operation (SO) group and ANP group. ANP was induced in rats by retrograde injection of 5% sodium taurocholate into the bili-pancreatic duct. Rats were killed at 3, 6, and 12 h after sodium taurocholate injection. Pancreatic histopathological changesand serum leve... |
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| Keywords: | Autophagy Acute necrotizing pancreatitis Microtubule-associated protein 1 light chain 3 Beclin-1 |
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