| 幽门螺杆菌对胃癌细胞BGC-823形态及凋亡相关基因表达的影响 |
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| 引用本文： | 吴莺,李翔,周红,范钰,张尤历,沈琰,何亚龙.幽门螺杆菌对胃癌细胞BGC-823形态及凋亡相关基因表达的影响[J].世界华人消化杂志,2011(17):1767-1772. |
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| 作者姓名： | 吴莺 李翔 周红 范钰 张尤历 沈琰 何亚龙 |
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| 作者单位： | 江苏大学附属医院消化科;江苏大学基础医学与医学技术学院;江苏大学附属人民医院肿瘤科; |
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| 基金项目： | 江苏省研究生培养创新计划基金资助项目,No.1221270009~~ |
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| 摘 要： | 目的:探讨H.pylori 刺激人胃癌细胞BGC-823 后,对细胞形态以及凋亡抑制基因Survivin和凋亡基因caspase-3 mRNA表达的影响.方法:分别用东亚型H.pylor i 菌株和西方型H.pylor i 菌株超声提取物,刺激胃癌细胞BGC-823,显微镜下直接观察细胞形态的变化.同时利用荧光定量聚合...
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| 关 键 词： | 幽门螺杆菌 蜂鸟表型 凋亡相关基因 CagA蛋白分型 |
Effect of H.pylori extract on cellular morphology and apoptosis-related gene expression in human gastric cancer BGC-823 cells |
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| Ying Wu,Xiang Li,You-Li Zhang,Yan Shen,Ya-Long He.Effect of H.pylori extract on cellular morphology and apoptosis-related gene expression in human gastric cancer BGC-823 cells[J].World Chinese Journal of Digestology,2011(17):1767-1772. |
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| Authors: | Ying Wu Xiang Li You-Li Zhang Yan Shen Ya-Long He |
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| Institution: | Ying Wu,Xiang Li,You-Li Zhang,Yan Shen,Ya-Long He,Department of Gastroenterology,the Affiliated Hospital of Jiangsu University,Zhenjiang 212001,Jiangsu Province,China Hong Zhou,Department of Clinical Laboratory and Hematology,Jiangsu University,Zhenjiang 212013,China Yu Fan,Cancer Institute,the Affiliated People's Hospital of Jiangsu University,Zhenjiang 212002,China |
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| Abstract: | AIM: To investigate the effect of H.pylori extract on cellular morphology and apoptosisrelated gene expression in human gastric cancer BGC-823 cells.METHODS: After BGC-823 cells were treated with ultrasonic extract of the east Asia type or the Western type of H.pylori strain, the changes in cellular morphology were observed by microscopy, and the expression of survivin and caspase-3 mRNAs was detected by fluorescent quantitative polymerase chain reaction (QRTPCR). The possible correlation between the expres... |
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| Keywords: | H pylori Hummingbird phenotype Apoptosis-related genes CagA typing |
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