耐甲氧西林金黄色葡萄球菌的检测及耐药性分析

目的 分析耐甲氧西林金黄色葡萄球菌的临床感染现状及耐药性.方法 采用美国MicroScan全自动细菌分析仪鉴定细菌种类,并用纸片扩散法进行药敏试验.结论 2006～2007年共分离出金黄色葡萄球菌270株,其中,耐甲氧西林金黄色葡萄球菌(MRSA)174株,检出率为64.4%.所有的分离株对万古霉素都敏感;MRSA对 红霉素、克林霉素、庆大霉素、复方磺胺、利福平和左旋氧氟沙星的耐药率分别为94.8%、71.8%、67.8%、85.1%、15.5%和48.9%.结论MRSA的分离率显著增高,耐药性严重,临床细菌室应高度重视MRSA的检测.

Analysis of methicillin-resistant Staphylococcus aureus to commonly used antibiotics

Objective To analyze the incidence and resistance of tnethicillin-resistant Staphylococcus aureus( MRSA). Methods The bacterial strains were automatically identified with MicroScan-Walk-Away-40, and then the antibiotic resistances were tested by standard Kirby-Bauer method. Results A total of 270 strains were isolated during 2006 -2007,in which 174 were MRSA(64.4% ). All isolates were susceptible to vancomycin. The resistant rate of the isolates of MRSA to erythromycin,clindamycin, gentamicin,sulfamethoxazole, rifampin and levofloxaxin were 94. 8% ,71. 8% ,67. 8% ,85. 1% , 15. 5% and 48. 9%. Conclusions The isolation rate of MRSA increased significantly. For effective supervision and control of Staphylococcus aureus infection, MRSA surveying should be included in clinical microbiology surveillence.