| 糙叶败酱提取物的体外抗肿瘤活性比较 |
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| 引用本文： | 陈茹,赵健雄,王学习.糙叶败酱提取物的体外抗肿瘤活性比较[J].四川中医,2011(1):50-52. |
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| 作者姓名： | 陈茹 赵健雄 王学习 |
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| 作者单位： | 广西中医学院护理学院;兰州大学中西医结合研究所; |
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| 摘 要： | 目的：比较糙叶败酱提取物糙叶败酱粗多糖、糙叶败酱总木脂素、糙叶败酱总皂苷的体外抗肿瘤活性,初步筛选糙叶败酱抗癌作用有效成分。方法：采用四甲基偶氮唑盐比色法（monotetrazolium test,MTT）测定不同浓度糙叶败酱粗多糖、糙叶败酱总木脂素、糙叶败酱总皂苷分别对人肺源腺癌细胞SPCA-1、人肝癌细胞系HepG2、和人慢性髓系白血病细胞K562生长的影响。结果：糙叶败酱总木脂素、总皂苷对体外培养的肿瘤细胞SPCA-1、HepG2、K562均有抑制作用,且抑制作用与药物浓度及作用时间有相关性;糙叶败酱总木脂素对K562细胞生长的抑制作用尤为显著,400μg/ml,作用96h,IC50为21.16μg/ml;糙叶败酱粗多糖对SPCA-1细胞的增殖有抑制作用。结论：糙叶败酱不同成分有不同的抗肿瘤作用,糙叶败酱总木脂素对人慢性髓系白血病细胞生长有明显的抑制作用。
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| 关 键 词： | 糙叶败酱 多糖 总木脂素 总皂苷 MTT法 |
Comparing the Differences among Patrinia scabra Bunge.Extractions Growth-inhibiting Effects of on Tumor Cell in Vitro |
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| Chen Ru Zhao jianxiong Wang Xuexi .Guangxi College of Nursing of Traditional Chinese Medicine.Comparing the Differences among Patrinia scabra Bunge.Extractions Growth-inhibiting Effects of on Tumor Cell in Vitro[J].Journal of Sichuan of Traditional Medicine,2011(1):50-52. |
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| Authors: | Chen Ru Zhao jianxiong Wang Xuexi Guangxi College of Nursing of Traditional Chinese Medicine |
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| Institution: | Chen Ru Zhao jianxiong Wang Xuexi 1.Guangxi College of Nursing of Traditional Chinese Medicine(Guangxi Nanni 530022,China) 2.Institute of the Combination of Traditional Chinese Medicine and Western Medicine in Lanzhou University(Lanzhou Gansu 730000,China) |
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| Abstract: | Objective：Comparing the differences among Patrinia scabra Bunge.extractions on growth-inhibiteng effects of tumor cell in vitro and testing anti-tumor effects of different Patrinia scabtra Bunge.extractions.Methods：The SPCA-1、HepG2、K562 cells growth was measured by MTT-microculture tetrazoliu assay.Results：Patrinia scabra Bunge.lignanoid、saponin inhibited the SPCA-1、HepG2、K562 cell growth in concentration-and time-dependent manners.And the effect of Patrinia scabra Bunge.lignanoid is more significant.IC50 is 21.16μg/ml after intervention in 400μg/ml Patrinia scabra Bunge.llignanoid for 96h,But polysaccharide has different effect on different tumor cell.Conclusions：The anti-tumor effect of different Patrinia scabra Bunge.extractions are distinguished from treating different tumor cell.The effect of Patrinia scabra Bunge liynanoid is more significant. |
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| Keywords: | Patrinia scabra Bunge lignanoid saponin polysaccharide monotetralium test(MTT) |
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