| SPE-HPLC-ELSD法测定三七药材中单糖和二糖的含量 |
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| 引用本文： | 高阳,牛涛,陈虹,徐波,孔令义.SPE-HPLC-ELSD法测定三七药材中单糖和二糖的含量[J].现代中药研究与实践,2012(2):29-31. |
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| 作者姓名： | 高阳 牛涛 陈虹 徐波 孔令义 |
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| 作者单位： | 中国药科大学天然药物化学教研室;天津天士力现代中药资源股份有限公司 |
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| 摘 要： | 目的建立高效液相色谱-蒸发光散射检测法,同时测定三七药材中单糖和二糖的含量。方法 HPLC采用Thermo Hypersil GOLDAmino氨基柱(250×4.6 mm),流动相为乙腈-水(75:25),流速0.8mL min-1,ELSD漂移管温度75℃,载气为空气,载气流速2.5 L min-1。结果在该条件下三七药材中的单糖和二糖组分能够得到较好的分离,线性关系良好(相关系数R2>0.99),回收率在98.23%~101.46%之间,且相对标准偏差小于3%。结论该方法简便、准确、灵敏、稳定,适合于三七药材中单糖和二糖的含量测定。
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| 关 键 词： | 三七 高效液相色谱-蒸发光散射检测 单糖 二糖 |
Determination of Monosaccharide and Disaccharide in Panax notoginseng by HPLC with SPE and ELSD |
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| GAO Yang,NIU Tao,CHEN Hong,XU Bo,KONG Ling-yi.Determination of Monosaccharide and Disaccharide in Panax notoginseng by HPLC with SPE and ELSD[J].Research and Practice on Chinese Medicines,2012(2):29-31. |
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| Authors: | GAO Yang NIU Tao CHEN Hong XU Bo KONG Ling-yi |
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| Institution: | 1(1.Department of Natural Medicinal Chemistry,China Pharmaceutical University,Nanjing 210009,China;2.Tianjin Tasly Modern TCM Resources co.,ltd.,Tianjin 300402,China) |
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| Abstract: | Objective An HPLC-ELSD method was established for the simultaneous quantitative analysis of glucose,fructose,sucrose from Panax notoginseng.Methods The chromatographic separation was achieved on an amino column using a mobile phase composed of a mixture of acetonitrile and water(75:25) at a flow rate of 0.8 mL/min by gradient elution.The ELSD drift tube temperature was set at 75 ℃ and the carrying gas was air.Results Under the above conditions,monosaccharides and disaccharides from Panax notoginseng could be well separated,and the spiked recoveries were 98.23%~101.46% with a RSD less than 3%(n = 3).Conclusion This method was accurate,rapid,simple and reproducible,and thus it would be suitable for the determination of monosaccharides,disaccharides from Panax notoginseng. |
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| Keywords: | Panax notoginseng HPLC-ELSD monosaccharide disaccharide |
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