XAGE-1b基因在非小细胞肺癌中的表达及与临床特点的相关性研究

目的 探讨XAGE-1b基因在非小细胞肺癌中的表达水平及与临床特点的相关性.方法 非小细胞肺癌患者30例,通过手术获得肺癌组织和癌旁正常肺组织,分别提取组织总RNA,RT-PCR反应扩增XAGE-1b基因全长,通过电泳和基因测序鉴定RT-PCR产物,确定基因表达阳性标本,分析基因表达阳性率及与临床特征相关性.结果 30例肺癌组织中,XAGE-1b基因表达阳性率为40%(12/30),30例癌旁正常肺组织不表达该基因;基因表达与患者年龄、性别、肿瘤分化程度无相关性,与病理类型有相关性,腺癌中基因表达阳性率明显高于其他病理类型(61.1%vs 8.3%,p=0.015);随着TNM分期的升高,基因表达阳性率略有增加,但差异无统计学意义(P＞0.05).结论 XAGE-1b基因在肺腺癌中高表达,可优先选择该基因作为肺腺癌免疫治疗靶点.

Association of XAGE-1b gene expression with clinical characteristics of non-small cell lung cancer

OBJECTIVE: To explore the association between XAGE-1b gene expression and the clinical characteristics of non-small cell lung cancer (NSCLC). METHODS: Tumor tissue and adjacent normal lung tissue specimens were obtained surgically from 30 patients with resectable NSCLC, from which the total RNA was extracted for RT-PCR to amplify full-length XAGE-1b gene. The products of RT-PCR were identified by electrophoresis and sequencing. The expression of XAGE-1b gene and its association with the clinical characteristics of the patients were analyzed. RESULTS: In the 30 tumor tissue specimens, the expression rate of XAGE-1b gene was 40%, but none of the normal lung tissues expressed this gene. The gene expression was not related to the patients' age, gender, tumor differentiation or clinical stages, but showed significant correlation to their pathological classification. The expression rate of XAGE-1b gene in adenocarcinoma was much higher than that in tumors of other pathological types (61.1% vs 8.3%, P=0.015). XAGE-1b gene expression tended to increase with the TNM stages, which, however, failed to find statistical data support (P>0.05). CONCLUSIONS: XAGE-1b gene is highly expressed in lung adenocarcinoma, and can be an ideal target for tumor immunotherapy.