| 细粒棘球绦虫Eg95抗原表位的生物信息学预测 |
| |
| 引用本文： | 李玉娇,王晶,赵慧,贾海英,李博,马秀敏,温浩,丁剑冰.细粒棘球绦虫Eg95抗原表位的生物信息学预测[J].中国人兽共患病杂志,2011(10):892-896,900. |
| |
| 作者姓名： | 李玉娇 王晶 赵慧 贾海英 李博 马秀敏 温浩 丁剑冰 |
| |
| 作者单位： | [1]新疆医科大学第一附属医院新疆重点实验室,乌鲁木齐830011 [2]新疆医科大学附属中医医院,乌鲁木齐830000 [3]新疆自治区人民医院,乌鲁木齐830000 [4]新疆医科大学基础医学院,乌鲁木齐830011 |
| |
| 基金项目： | 国家自然科学基金项目（30901374 81060135 30560146 30860263）资助 |
| |
| 摘 要： | 目的应用软件和在线网络分析Eg95的蛋白二级结构,预测B细胞表位和T细胞表位,为确定和筛选优势表位,研制安全、高效的表位疫苗奠定基础。方法采用DNAStar软件和在线网站IEDB、SYFPEITHI等对Eg95的B细胞表位和T细胞表位进行预测。结果 Eg95存在潜在的抗原表位,B细胞表位区域：16-38,41-48,50-67,68-90,92-100,103-113,120-132。分值高的T细胞表位区域：6-14,14-22,48-56,4-12,98-106,142-150,146-154。结论运用生物信息学方法确定Eg95存在7个抗原表位,对进一步研究Eg95的抗原性和研发优势表位疫苗具有重要的意义。
|
| 关 键 词： | Eg95 抗原表位 生物信息学 |
Bioinformatics prediction on Eg95 antigen epitopes of Echinococcus granulosus |
| |
| LI Yu-jiao,WANG Jing,ZHAO Hui,JIA Hai-ying,LI Bo,MA Xiu-min,WEN Hao,DING Jian-bing.Bioinformatics prediction on Eg95 antigen epitopes of Echinococcus granulosus[J].Chinese Journal of Zoonoses,2011(10):892-896,900. |
| |
| Authors: | LI Yu-jiao WANG Jing ZHAO Hui JIA Hai-ying LI Bo MA Xiu-min WEN Hao DING Jian-bing |
| |
| Institution: | (Medical Research Center and Xinjiang Key First,First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China) |
| |
| Abstract: | The protein secondary structure was analyzed by TMHMM Sever2.0 and SOPMA Sever,and B cell and T cell epitopes of Eg95 were predicted by DNAStar,IEDB and SYFPEITHI.Many distinct antigenic epitopes of Eg95 were identified by computation,and the possible B cell epitopes of which were in the regions of 16-38,41-48,50-67,68-90,92-100,103-113,and 120-132.The high score of T cell epitopes were in the regions of 6-14,14-22,48-56,4-12,98-106,142-150,and 146-154.These results indicated that there are 7 antigen epitopes in Eg95 antigen determined by bioinformatics methods,which are significant in the further research of Eg95 antigenic and development of dominant epitope vaccine. |
| |
| Keywords: | Eg95 antigen epitope bioinformatics |
| 本文献已被 维普 等数据库收录！ |
|
