丹栀逍遥散对D-氨基半乳糖/脂多糖诱导的大鼠急性肝损伤保护作用机制研究

目的 探讨丹栀逍遥散对D-氨基半乳糖(D-GaIN)/脂多糖(LPS)诱导的大鼠急性肝损伤(ALI)的保护作用及其可能的作用机制.方法 将110只SD大鼠随机分为对照组、模型组、小剂量、中剂量和大剂量丹栀逍遥散干预组,每组22只.采用D-GaIN)/LPS腹腔注射建立ALI模型,分别给予生理盐水或药物灌胃干预.采用EL...

Protective mechanism of Danzhi Xiaoyao powder on hepatic damage in rats with D-galactosamine/lipopolysaccharides-induced acute liver injury

Objective The aim of this study was to explore the protective mechanism of Danzhi Xiaoyao powder on hepatic damage in rats with D-galactosamine(D-GaIN)/lipopolysaccharide(LPS)-induced acute liver injury(ALI).Methods 110 SD male rats were randomly divided into control,model,low-dose,moderate dose and large-dose of herbal medicine-intervened groups,with 22 rats in each.The ALI model was built-up by D-GaIN/LPS injection intraperitoneally,and the herbal medicine was given by lavage.Serum MDA,SOD,GSH-PX,IL-6,IL-1β,TNF-αlevels were detected by ELISA,and serum HA,LN andⅢ-PC levels were assayed by RIA.Results Serum AST,ALT,bilirubin and MDA levels in model group were(12081.3±587.7)U/L,(7658.7±742.2)U/L,(104.3±10.3)μmol/L and(8.9±1.1)mmol/mL,all significantly higher than[(171.9±17.2)U/L,(60.5±6.2)U/L,(2.4±0.2)μmol/L and(2.5±0.3)mmol/mL,respectively,P<0.05]in the control;serum SOD and GSH-PX levels were(14.3±1.7)U/mL and(7.1±0.7)μmol/L,both significantly lower than[(38.6±4.4)U/mL and(21.9±2.2)μmol/L,P<0.05]in the control,while serum SOD and GSH-PX levels in three herbal medicine-intervened groups significantly higher than those in the model(P<0.05);serum IL-6,IL-1βand TNF-αlevels in the model were(194.3±20.5)ng/mL,(24.7±2.5)ng/ml and(864.5±87.3)ng/mL,significantly higher than[(90.1±10.3)ng/mL,(5.2±1.0)n g/mL and(30.2±4.1)ng/mL in the control,while all significantly decreased compared to those in the model in three herbal medicine-intervened groups(P<0.05);serum HA,LN andⅢ-PC levels were(286.3±29.1)ng/ml,(297.4±30.5)ng/ml and(257.1±26.7)ng/ml,all significantly higher than[(55.1±6.2)ng/mL,(75.1±7.6)ng/mL and(61.9±6.2)ng/mL]in the control,while all significantly decreased compared to those in the model in three-herbal medicine-intervened groups(P<0.05).Conclusion Danzhi Xiaoyao powder could exert significant protective effect on liver functions in rats with D-GaIN/LPS-induced ALI,and the mechanism by which it works might be related to obvious inhibition of oxidative stress and inflammation response.