原发性肝癌患者外周血CD4+T、CD8+T、Tc17、Th17和Treg淋巴细胞的变化及其意义

目的 了解原发性肝癌(PLC)患者外周血CD4+T、CD8+T、Tc17、Th17和Treg淋巴细胞的变化.方法 2018年6月～2019年12月我院诊治的PLC患者83例(巴塞罗那临床肝癌分期A期25例,B期23例,C期18例,D期17例)和健康人35例,采用流式细胞技术检测外周血CD4+T、CD8+T及Tc17(C...

Implication of peripheral CD4+T,CD8+T,Tc17,Th17 and Treg lymphocyte changes in patients with primary liver cancer

Objective The aim of this study was to investigate the implication of peripheral CD4⁺T,CD8⁺T,Tc17,Th17 and Treg lymphocyte changes in patients with primary liver cancer(PLC).Methods 83 patients with PLC(BCLC stage A in 25,stage B in 23,stage C in 18,and stage D in 17)and 35 healthy individuals were recruited in our hospital between June 2018 and December 2019,and the percentages of peripheral blood CD4⁺T,CD8⁺T,Tc17(CD8⁺IL-17),Th17(CD4⁺IL-17)and CD4⁺CD25⁺CD45RA⁺Treg lymphocytes were detected by flow cytometry.Results The percentage of peripheral blood CD4⁺T lymphocytes in patients with PLC was(32.8±8.5)%,significantly lower than[(43.3±7.4)%,P<0.05],and the ratio of CD4⁺/CD8⁺cells were(0.9±0.3),also significantly lower than[(1.2±0.1),P<0.05]in healthy control;the percentages of peripheral blood Treg cell[(6.4±0.9)%vs.(3.0±0.1)%],Th17 cell[(5.0±1.1)%vs.(3.1±1.5)%]and Tc17 cell[(2.3±0.4)%vs.(1.0±0.2)%]were all significantly increased as compared to those in the control(all P<0.05);the percentage of CD4⁺CD25⁺CD45RA⁺Treg cell in patients with BCLC stage C/D increased greatly compared to that in patients with stage A/B[(7.6±0.4)%vs.(5.3±0.5)%,P<0.001],the percentages of Th17(CD4⁺IL-17)cell and Tc17(CD8⁺IL-17)cell also increased greatly compared to in patients with stage A/B[(6.9±1.1)%vs.(5.4±0.6)%,P<0.01 and(2.8±0.6)%vs.(1.6±0.4)%,P<0.01].Conclusion There is an immune imbalance of peripheral blood lymphocyte subsets in patients with PLC,which might hint the immunotherapy targeting at regulating T lymphocyte imbalance necessary in this setting.