|
|||||
|
|
| 红花多糖对人PBMC增殖活性及NK、LAK细胞杀伤活性的影响 | |
| 引用本文： | 周明瑶,阮殿清,陈德欣,石学魁,梁颖,王亚贤.红花多糖对人PBMC增殖活性及NK、LAK细胞杀伤活性的影响[J].中医药信息,2010,27(1):47-49. |
| 作者姓名： | 周明瑶 阮殿清 陈德欣 石学魁 梁颖 王亚贤 |
| 作者单位： | 1. 哈尔滨市卫生学校,黑龙江,哈尔滨,150010 2. 黑龙江省木兰县疾病预防控制中心,黑龙江,木兰,151900 3. 黑龙江中医药大学附属第一医院,黑龙江,哈尔滨,150040 4. 黑龙江中医药大学,黑龙江,哈尔滨,150040 |
| 摘 要： | 目的:探讨红花多糖(Safflower polysaccharide,SPS)体外对人外周血单个核细胞(PBMC)的增殖作用及对NK细胞、LAK细胞杀伤活性的影响。方法:采集健康成人外周血,常规分离PBMC,体外与不同浓度的SPS共同培养,用3H-TdR法检测PBMC的增殖活性;MTT法检测NK、LAK细胞的杀伤活性。结果:SPS对PBMC增殖有促进作用,其促增殖作用呈现量效依赖性,以1.25mg.mL-1和0.625mg.mL-1剂量组促增殖作用明显(P0.05);SPS对NK细胞与LAK细胞的杀伤活性都有明显的提升作用(P0.05)。结论:SPS可促进PBMC的增殖,增强NK细胞、LAK细胞的杀伤活性,增强免疫功能。 |
| 关 键 词： | 红花多糖 人外周血单个核细胞 增殖 NK细胞 LAK细胞 |
The effect of SPS on the proliferation of PBMC and the cytotoxicity of NK cells,LAK cells of PBMCs |
|
| ZHOU Ming-yao,YUAN Dian-qing,CHEN De-xin,SHI Xue-kui,LIANG Ying,WANG Ya-xian.The effect of SPS on the proliferation of PBMC and the cytotoxicity of NK cells,LAK cells of PBMCs[J].Information on Traditional Chinese Medicine,2010,27(1):47-49. | |
| Authors: | ZHOU Ming-yao YUAN Dian-qing CHEN De-xin SHI Xue-kui LIANG Ying WANG Ya-xian |
| Institution: | ZHOU Ming-yao1,YUAN Dian-qing2,CHEN De-xin3,SHI Xue-kui4,LIANG Ying4,WANG Ya-xian(1.Harbin Health School,Harbin 150010,China,2.Mulan County Centers for Disease Prevent , Control,Mulan 151900,3.The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,4.Heilongjiang University of Chinese Medicine,China) |
| Abstract: | Objective：To observe the effect of SPS on the proliferation of PBMC in vitro and the eytotoxieity of NK cells,LAK cells of PBMCs. Methods：PBMC from healthy donors were incubated with different doses of SPS in vitro. The proliferative activity of PBMC was tested by 3 H - TdR assay and the cytotoxieity of NK cells, LAK cells of PBMCs were measured by MTT assay. Resuhs：SPS could accelerate the proliferation of PBMC and its proliferation showed dose- dependent and the effect was obvious at 1.25mg · mL^-1 and 0. 625mg · mL^-1 dose group （ P 〈 0. 05 ） ; SPS could obviously promote the cytotoxieity of NK cell and LAK cell （ P 〈 0. 05 ）. Conclusion ： SPS can promote the proliferation of PBMC and enhance the eytotoxieity of NK cells, LAK cells and immune function. |
| Keywords: | Safflower polysaccharide(SPS) Peripheral blood mononuclear cells(PBMC) proliferation NK cell LAK cell |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |