甲醛吸入对小鼠的氧化损伤作用

目的　研究甲醛吸入对小鼠肝脏、脾脏、胸腺的过氧化损伤。方法　选用健康昆明种纯系小鼠 30只 ,随机分成 5组(即阴性对照组 ,1mg/m3 ,3mg/m3 ,5mg/m3 染毒组和阳性对照组 ) ,每组 6只 ,用静式吸入染毒方式染毒甲醛 (FA)。于染毒14d后 ,测定小鼠肝脏、脾脏、胸腺中超氧化物歧化酶 (superoxidedismutase,SOD)活性和丙二醛 (maleicdialdehyde,MDA)含量。结果　随甲醛染毒剂量的增高 ,小鼠肝脏、脾脏和胸腺SOD活性呈下降趋势 ,并且有明显的剂量—效应关系 ,1mg/m3 染毒组胸腺SOD活性与阴性对照组比较差异无显著性 ,其他各组与阴性对照组比较均有显著性差异 (P <0 0 5或P <0 0 0 1) ;MDA含量各染毒组均显著高于阴性对照组 (P <0 0 5或P <0 0 0 1)。结论　气态甲醛对肝脏、脾脏、胸腺均有氧化损伤作用 ,其分子机制涉及对SOD的抑制作用

Oxidative damage induced by formaldehyde inhalation in mice

Objective To study the oxidative damage induced by formaldehyde inhalation to liver, spleen and thymus of mice. Methods Thirty KM mice were randomly divided into 5 groups: negative control, 1 mg/m 3,3 mg/m 3,5 mg/m 3 FA inhalation and positive control groups. Mice were exposed to FA by inspiration in a static total enclosure chamber for 14 days. The activities of superoxide dismutase (SOD) and the contents of maleic dialdehyde (MDA) in liver,spleen and thymus were determined. Results A significant decline tendency of the activities of SOD was showed with increasing of doses.The results showed a significant doses-effect relationship.There was significant difference in all FA inhalation groups compared with negative control group (P<0.05 or P<0.001) except the 1 mg/m 3 group in thymus. The contents of MDA were increased significantly in all FA inhalation groups compared with negative control group (P<0.05 or P<0.001). Conclusion The gaseous formaldehyde reveales significant oxidation damage to liver,spleen and thymus of mice,and the molecular mechanism may be involved in the SOD activity repression.