DHMEQ对降植烷诱导的狼疮性肾炎的作用及机制研究

目的：探讨NF-κB抑制剂DHMEQ对降植烷诱导狼疮性肾炎小鼠模型的作用。方法方便选取2012年7月＿2015年12月用降植烷诱导形成狼疮性肾炎动物模型24例,研究DHMEQ对该模型的治疗效果,探讨DHMEQ对狼疮性肾炎的影响。结果 DHMEQ拮抗升高的抗核小体,抗dsDNA抗体和抗组蛋白自身抗体以及IL-1β,IL-6和IL-17以及TNF-α起到拮抗作用。DHMEQ可降低降植烷诱发的肾脏病变。磷酸化p38促分裂原活化蛋白激酶(MAPK),磷酸化c Jun氨基末端激酶(JNK﹚和NFκBp65的肾表达水平(MAPK﹚显著下调。结论 DHMEQ通过调节细胞因子水平和MAPK/JNK/NFκB信号通路对降植烷诱导狼疮性肾炎产生有利影响。

Research on DHMEQ Ameliorates Pristane induced Lupus in Mice

Objective The aim of the present study was to identify whether a NF-κB inhibitor, DHMEQ, ameliorates lupus nephritis in a pristane-induced mouse model. Methods Convenient selection from July 2012 to December 2015, 24 cases of lupus nephritis were induced by animal model, and the therapeutic effect of DHMEQ on the model was studied, and the ef-fect of DHMEQ on lupus nephritis was studied. Results DHMEQ was shown to antagonize the increasing levels of anti-nu-cleosome, anti-dsDNA and anti-histone autoantibodies, as well as the increasing levels of IL-1β, 6 and 17 and TNF-α. In addition, DHMEQ reduced the number of renal lesions caused by pristane, as reflected by milder proteinuria and reduced renal pathology. The renal expression levels of phosphorylated-p38 mitogen-activated protein kinase(MAPK), phosphorylat-ed-c-Jun N-terminal kinase (JNK) and NF-κB p65 were signifcantly downregulated. Conclusion The results of the present study indicate that DHMEQ has a beneficial effect on pristane-induced lupus through regulating cytokine levels and the MAPK/JNK/NF-κB signaling pathway.