3.0T MR成像对兔腹主动脉粥样硬化斑块破裂及血栓形成模型的成像研究

目的 探讨3.0T高分辨MRI对兔腹主动脉粥样硬化模型药物诱发斑块破裂和血栓形成的成像研究.方法 20只雄性新西兰白兔,采用数字表法随机分为实验组16只,对照组4只,采用间断高脂饲料喂养结合球囊拉伤腹主动脉技术建立动脉粥样硬化模型,并在建模3个月后给予蝰蛇毒+组胺药物诱发试验,以期斑块破裂和形成血栓.在药物诱发试验前后...

Detection of thrombosis and plaque rupture in atherosclerotic rabbit model by using 3.0 T MR imaging

Objective To explore the imaging of the thrombosis after pharmacological triggering of plaque rupture in atherosclerotic rabbit model by using 3.0 T high-resolution magnetic resonance imaging.Methods Twenty male New Zealand white rabbits were divided into an experimental group (n = 16) and a control group (n = 4).The aortic wall injuries were induced by an intravascular balloon in experimental group rabbits after high cholesterol diet.The pharmacological triggering with Russell's viper venom and histamine was performed after 3 months of establishment of model.All of the animals underwent pre-trigger and post-trigger MR examinations including 3D time of fight (3D TOF),T1 WI,T2WI and post contrast T1 WI.Euthanasia was performed in all rabbits and gross anatomy and histological specimen of aorta were obtained.Comparing the location and length of the thrombus between MRI images and histopathology was used Pearson test.Comparing the calculated indexes of abdominal aorta between rabbits with and without thrombosis was used AVONA test and LSD-t test.Results After triggering,8 in 14 survived rabbits developed thrombosis in experimental group,meanwhile,no thrombus was found in control group.The accuracy of multi-sequences MRI for detecting of thrombus was 87.1％ (27/31).MRI data correlated with the histopathology regarding thrombus length ( r = 0.85,P ＜ 0.01 ) and thrombus location ( r = 0.94,P＜0.01 ).Compared with rabbits without thrombosis,the rabbits with thrombosis had narrower lumen of abdominal aorta in the pre-triggered MR images [ ( 5.71 ± 2.38 )mm2 vs.( 8.93 ± 5.36) mm2,P ＜ 0.01 ].Conclusion MRI is useful tool to determine the thrombosis and plaque rupture in atherosclerotic rabbit model.