多发性肌炎和皮肌炎血清抗Jo-1抗体水平与疾病活动度相关性研究

目的 探讨抗Jo-1抗体水平与多发性肌炎/皮肌炎疾病活动度的相关性.方法 采用免疫印迹法和酶联免疫吸附试验(ELISA)法检测148例多发性肌炎/皮肌炎患者和性别年龄相匹配的130名健康人血清抗Jo-1抗体水平,利用国际肌炎组织制定的炎性肌病评价工具评估抗Jo-1抗体阳性患者疾病活动度(VAS),并分析抗Jo-1抗体水平与多发性肌炎肢肌炎疾病活动度的相关性.统计学处理采用x2检验或t检验.结果 免疫印迹法和ELISA法检测多发性肌炎/皮肌炎患者血清抗Jo-1抗体的阳性率分别是24.3％(36/148)和27.0％(40/148),健康人血清中抗Jo-1抗体均为阴性.发热、肺间质病变(ILD)和关节炎/关节痛在抗Jo-1抗体阳性组的比例明显高于抗Jo-1抗体的阴性组(P＜0.05).抗Jo-1抗体阳性患者的总疾病活动度评分与血清抗Jo-1抗体的浓度呈正相关(r=0.874,P=0.000).7例患者治疗后患者血清抗Jo-1抗体浓度与疾病活动度评分均显著下降,两者变化一致.结论 ELISA法检测抗Jo-1抗体水平与疾病活动相关,可作为多发性肌炎/皮肌炎疾病活动的评价指标.

Serum anti-Jo-1 antibody level correlates with disease activity in polymyositis and dermatomyositis

Objective To evaluate the correlation between serum anti-Jo-1 antibody level and myositis disease activity in polymyositis/dermatomyositis.Methods Anti-Jo-1 antibody levels in serum from 148 polymyositis/dermatomyositis patients and 130 healthy controls were measured by both ELISA and immunoblot assay.Disease activity of the anti-Jo-1 antibody positive patients was assessed by the global myositis disease activity score (visual analogue score,VAS) established by the International Myositis Assessment and Clinical Studies (IMACS) Group.The correlation between disease activity and level of serum antiJo-1 antibody was assessed.Comparisons between groups were performed by x2 test or t test.Results The positive rate of ELISA and EUROLINE was 24.3％(36/148) and 27.0％(40/148) respectively.Fever,ILD,arthritis/arthralgia were found to be more predominant in anti-Jo-1 antibody positive patients than those who were anti-Jo-1 antibody negative patients.There was significant positive correlation between the global myositis disease activity score (VAS) and serum level of anti-Jo-1 antibody (r=0.874,P=9.000).Serum antiJo-1 antibody levels,together with global disease activity,were significantly decreased in the 7 patients after treatment.Conclusion Anti-Jo-1 antibody level measured by ELISA is associated with disease activity of PM/DM,and could be a marker of disease activity.