兔踝关节外侧韧带Brostr(o)m重对韧带本体感觉的影响

目的探讨兔踝关节外侧韧带Brostr(o)m重建术对韧带本体感觉的影响。方法取63只成年新西兰大白兔，随机选取9只处死后切取踝关节外侧韧带(空白对照组)；余54只全部切断踝关节外侧韧带，随机选取其中27只行Brostr(o)m重建术(实验组)，27只旷置韧带(实验对照组)。术后第2、6和12周，两个实验组分别随机选取9只动物，处死后切取踝关节外侧韧带，行改良O'Connor and Gonzales氯化金染色后于光镜下观察、计数本体感受器小体。结果在踝关节外侧韧带内找到并识别了四种机械感受器：Ruffini小体、Pacini小体、Golgi腱器官及游离神经末梢。对前三种进行计数，空白对照组机械感受器小体总数为(21.0±3.5)个；实验组术后2、6、12周分别为(12.7±2.1)、(13.0±3.0)、(16.0±2.0)个；实验对照组术后2、6、12周分别为(7.7±1.5)、(4.7±1.5)、(6.3±0.6)个。实验组术后2，6，12周之间的差异有统计学意义(F=7.53，P=0.00)，随时间推移小体数逐渐增多。实验对照组类似(F=16.27，P=0.00)。术后2、6、12周三组间的差异有统计学意义(F=88.75，102.91，122.53，P=0.00)，实验组较实验对照组多，较空白对照组少。结论兔踝关节外侧韧带断裂后，不论手术与否，均会出现机械感受器小体数量下降；Brostr(o)m解剖重建术较韧带旷置能保留更多的机械感受器小体。

The effect on the proprioception after Brostr(o)m reconstruction for lateral ankle ligament in rabbit

Objective To study the effect on the proprioception after Brostr(o)m reconstruction for lateral ankle ligament in rabbit. Methods Six-three adult New Zealand white rabbits were used in this study. Nine rabbits were random selected as the blank control group, these rabbits were sacrificed and the lateral ankle ligaments were taken. The rest were all cut the lateral ankle ligament. Twenty-seven rabbits were performed Brostr(o)m reconstruction as the experimental group, and the other were left the apocoptic ligament as the experimental control group. At 2, 6, and 12 weeks after operation, 9 rabbits of each experimental group were sacrificed, and the lateral ligament of ankle were taken randomly for observation. All the lateral ligaments of ankle were stained with a modification of gold-chloride method. We counted the mechanoreceptors under light microscope. Results We identified the four mechanoreceptors: Ruffini receptors, Pacini receptors, Golgi tendon organ-like receptors and free nerve endings, and the first three of them were counted.The amount of mechanoreceptors in blank control group was 21.0±3.5, in experiment group at 2, 6, and 12weeks after operation were 12.7±2.1, 13.0±3.0, and 16.0±2.0 respectively, and in experiment control group was 7.7±1.5, 4.7±1.5, and 6.3±0.6 respectively. The difference among the 2, 6 and 12 weeks after operation in experiment group were statistically significant (F=7.53, P=0.00) and similarly in experiment control group (F=16.27, P=0.00), as time goes on, the amount of mechanoreceptors were increasing gradually. The difference among three group at the 2, 6 and 12 weeks after operation separately were statistically significant (F=88.75, 102.91, 122.53, P=0.00), the amount of mechanoreceptors in experiment group were more than in experiment control group, and lesser than in blank control group. Conclusion After rupture of the lateral ankle ligament of rabbit, whether operation or not, the ligament's mechanoreceptors will be decreased. Brostr(o)m anatomical reconstruction for the ruptured ligament can retain the maximum amount of mechanoreceptors.