复方丹参健力浓缩颗粒对模拟高强度海训小鼠抗运动性疲劳的实验研究

目的 研究复方丹参健力浓缩颗粒对模拟高强度海训小鼠抗运动性疲劳的药理作用.方法 100只昆明种小鼠按数字表法随机分为5组:空白对照组、阳性对照组及复方丹参健力浓缩颗粒低、中、高(72、216、864 mg/kg)剂量组,每组20只,灌胃给药14 d后分别观察小鼠负重游泳时间及血乳酸、尿素氮、肝糖原等生化指标的变化.结果 复方丹参健力浓缩颗粒能显著延长小鼠负重游泳时间[ (486±168)、(596±145)、(646±116)s],血乳酸的廓清率[(39.30±2.28)、(48.60±3.26)、(54.10±1.66) μmol/(L·min)]、肝糖原[(27.36±6.24)、(33.62±4.76)、(37.34±5.54) mg/g]含量明显增加,而血清尿素氮[(11.12±1.60)、(10.38±1.50)、(10.06±1.00) mmol/L]含量明显降低,差异有统计学意义(P＜0.05或0.01).结论 复方丹参健力浓缩颗粒具有显著的抗运动性疲劳作用,是一种有效抗运动性疲劳的中药制剂.

Experimental research on the effect of compound Danshen Jianli granule on exertional fatigue induced by simulated high intensity training at sea in mice

Objective To investigate the pharmacological effect of compound Danshen Jianli granule on exertional fatigue induced by simulated high intensity training at sea in mice.Methods One hundred KM mice were randomly divided into 5 groups:the control group,the positive control group,and the low,mid and high dosage groups (72,216,864 mg/kg),each consisting of 20 animals.Fourteen days after gastrogavage,the duration of swimming time,and changes in such biochemical data as blood lactate,urea nitrogen and hepatic glycogen were detected respectively.Results Compound Danshen Jianli granule could significantly prolong the duration of the mice swimming with load [ (486 ± 168 ),(596 ± 145 ),(646 ± 116)s ].Clearance of blood lactate [ (39.3 ± 2.28 ),(48.6 ± 3.26 ) and ( 54.10 ± 1.66 ) μmol/( L · min) ],levels of hepatic glycogen [ (27.36 ± 6.24 ),(33.62 ± 4.76),( 37.34 ± 5.54 ) mg/g ] were increased obviously,while contents of urea nitrogen [ ( 11.12 ± 1.60),( 10.38 ± 1.50),( 10.06 ± 1.00) mmol/L] was decreased,all with statistical significance (P ＜0.05 or 0.01 ).Conclusions Compound Danshen Jianli granule has an obvious effect on exertional fatigue and is a Chinese medicinal preparation effective on exertional fatigue.