MCM2和Ki-67及Rb蛋白在喉鳞状细胞癌中的表达及意义

目的:探讨微染色体维持蛋白2(MCM2)、增殖细胞核抗原(Ki-67)和视网膜母细胞瘤易感基因(Rb)与人喉鳞状细胞癌(LSCC)发生、发展和预后的关系。方法:应用免疫组织化学ElivisionTMplus二步法检测60例LSCC、10例喉癌前病变、10例声带息肉及10例癌周正常喉组织中MCM2与Rb蛋白的表达,同时检测Ki-67蛋白在LSCC及癌周正常喉组织中的表达并结合临床资料进行分析。结果:①LSCC中MCM2蛋白的表达水平明显高于喉癌前病变及癌周正常喉组织(均P<0.05),且与喉癌病理分级、临床分期及淋巴结转移有关(均P<0.05);LSCC中Rb蛋白表达水平明显低于癌前病变及癌周正常喉组织(均P<0.05);LSCC组织中MCM2与Rb蛋白的表达呈负相关(r=-0.542,P<0.05);②LSCC组织中Ki-67蛋白的阳性表达率(76.67%)明显高于癌周正常喉组织(30.00%),差异有统计学意义(P<0.01),MCM2、Ki-67蛋白在LSCC中的表达呈正相关(r=0.596,P<0.01),MCM2与Ki-67蛋白的标记指数在生存时间≥3年组和<3年组差异有统计学意义(P<0.05)。结论:MCM2蛋白的表达升高和Rb蛋白的表达下降与LSCC的发生、发展有关,检测MCM2有助于判定LSCC的恶性程度及预后。

Expression and significance of MCM2, Ki-67 and Rb protein in laryngeal squamous cell carcinomas

Objective:To investigate the expression of MCM2,Ki-67 and Rb and its biological characteristic in human laryngeal squamous cell carcinomas(LSCC).Method:The expression of MCM2 protein and Rb protein were detected in 60 cases of LSCC,10 cases of precarcinoma,10 cases of vocal cord polyps and 10 cases of normal laryngeal tissues,and Ki-67 protein were detected in 60 cases of LSCC and 10 cases of normal laryngeal tissues by Elivision TM plus immunohistochemical staining,and analyze their relations with clinicopathological characteristics.Result:The positive expression rate of MCM2 in LSCC was significantly higher than that in precarcinoma and normal laryngeal tissues(P<0.05),and was positively correlated with pathological grades,clinical stages and lymph node metastases(P<0.05) of LSCC.The positive expression rate of Rb protein in LSCC was significantly lower than that in precarcinoma and normal laryngeal tissues(P<0.05).The expression level of MCM2 in LSCC was negatively corelated with Rb(r=-0.542,P<0.05),the expression level of Ki-67 in LSCC(76.67%)was significantly higher than that in normal laryngeal tissues(30.00%)(P<0.01) and the expression level of MCM2 in LSCC was positively corelated with Ki-67(r=0.596,P<0.01).The LI of MCM2 in the 3-year survival rate of LSCC was significantly lower than that in Ki-67(P<0.05).Conclusion:Over expression of MCM2 and loss of Rb protein were related to the carcinogenesis and development of LSCC.The determination of MCM2 can be an index for estimating the level of malignancy and prognosis of LSCC.