Pre-treatment with bone marrow-derived mesenchymal stem cells inhibits systemic intravascular coagulation and attenuates organ dysfunction in lipopolysaccharide-induced disseminated intravascular coagulation rat model |
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Authors: | Wang Biao Wu Shu-Ming Wang Tao Liu Kai Zhang Gong Zhang Xi-Quan Yu Jian-Hua Liu Chuan-Zhen Fang Chang-Cun |
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Institution: | Department of Cardiovascular Surgery, Qilu Hospital, Shandong University, Jinan, Shandong 250012, China. |
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Abstract: |
Methods Forty-eight adult male rats were divided into five experimental groups and one control group with eight animals in each group. In the treatment groups, 0, 1´10 6, 2´10 6, 3´10 6, and 5´10 6 of BMSCs were injected intravenously for 3 days before LPS injection, while the control group was treated with pure cell culture medium injection. Then, the LPS (3 mg/kg) was injected via the tail vein in the treatment groups, while the control group received 0.9% NaCl. Blood was withdrawn before and 4 and 8 hours after LPS administration. The following parameters were monitored: platelets (PLT), fibrinogen (Fib), D-dimer (D-D), activated partial thromboplastin time (APTT), prothrombin time (PT), tumor necrosis factor-a (TNF-a), interferon-g (IFN- g), interleukin- 1b (IL-1b), creatinine (Cr), alanine aminotransferase (ALT), creatinine kinase-MB (CK-MB), and endothelin (ET).
Results Compared with the control group, a significant change of coagulation parameters were found in the experimental groups. The plasma level of the inflammatory mediator (TNF-a, IFN-g, IL-1b), organ indicator (Cr, ALT, and CK-MB), and ET in the experimental groups were much lower ( P <0.05) than that in the control group. Furthermore, some of these effects were dose-dependent; the statistical comparison of the plasma levels between the groups (from group 2 to group 5) showed a significant difference ( P <0.05), except the ALT and CK-MB levels ( P >0.05).
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Keywords: | disseminated intravascular coagulation lipopolysaccharide mesenchymal stem cells proinflammatory cytokine |
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