| 人PTN基因在脑胶质瘤、肝细胞癌、喉鳞癌中的表达研究 |
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| 引用本文： | 陈菊祥,卢亦成,唐榕,方雄英,夏放,廖建春,楼美清,付志仁,应康,谢毅,毛裕民. 人PTN基因在脑胶质瘤、肝细胞癌、喉鳞癌中的表达研究[J]. 第二军医大学学报, 2001, 22(7): 628-630,F003 |
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| 作者姓名： | 陈菊祥 卢亦成 唐榕 方雄英 夏放 廖建春 楼美清 付志仁 应康 谢毅 毛裕民 |
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| 作者单位： | 第二军医大学长征医院神经外科,;上海联众基因科技研究院;第二军医大学长征医院耳鼻咽喉科;长征医院普通外科 |
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| 基金项目： | 国家自然科学基金资助项目 ( 3 9770 792 ) |
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| 摘 要： | 目的：运用基因表达谱芯片和原位杂交技术观察人PTN（pleiotrophin) 基因在某些恶性肿瘤中的表达,并探讨其在肿瘤发生发展中的作用。方法：用BioDoor 4096型cDNA基因表达谱芯片和原位杂交技术检测PTN基因在5例脑胶质瘤、10例喉鳞癌、6例肝细胞癌及正常对照组织中的表达水平。结果：在上述肿瘤的基因表达谱芯片检测中,PTN基因表达均显著增高,与脑胶质瘤、喉鳞癌、肝细胞癌的原位杂交检测结果相吻合。结论：cDNA基因表达谱芯片能够为恶性肿瘤的相关基因功能分析提供特异和可靠的数据。PTN基因在恶性肿瘤的发生机制中可能起重要作用。
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| 关 键 词： | PTN基因 基因芯片 原位杂交 基因表达调控 肿瘤 病因学 |
| 文章编号： | 0258-879(2001)07-0628-03 |
Expression of PTN gene in glioma, hepatoma and laryngeal squamous cell carcinoma |
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| CHEN Ju Xiang ,LU Yi Cheng ,TANG Rong ,FANG Xiong Ying ,XIA Fang ,LIAO Jian Chun ,LOU Mei Qing ,FU Zhi Ren ,YING Kang ,XIE Yi ,MAO Yu Min. Expression of PTN gene in glioma, hepatoma and laryngeal squamous cell carcinoma[J]. Former Academic Journal of Second Military Medical University, 2001, 22(7): 628-630,F003 |
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| Authors: | CHEN Ju Xiang LU Yi Cheng TANG Rong FANG Xiong Ying XIA Fang LIAO Jian Chun LOU Mei Qing FU Zhi Ren YING Kang XIE Yi MAO Yu Min |
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| Affiliation: | CHEN Ju Xiang 1,LU Yi Cheng 1,TANG Rong 2,FANG Xiong Ying 3,XIA Fang 2,LIAO Jian Chun 4,LOU Mei Qing 1,FU Zhi Ren 5,YING Kang 2,XIE Yi 2,MAO Yu Min 2 |
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| Abstract: | Objective:To investigate the role of pleiotrophin (PTN) gene in carcino genesis using cDNA microarray and in situ hybridization. Methods:The expression of PTN gene in 5 cases of glioma, 10 laryngeal squamous cell carcinoma, 6 cases of hepatocarcinoma, and normal controls were detected by BioDoor 4096 type cDNA microarray and in situ hybridization. Results: The expression of PTN gene in carcinoma samples were significantly higher than in normal controls by cDNA microarray, the results was the same as by in situ hybridization. Conclusion: cDNA microarray is an effective technique in analysis of functional study of associated genes in carcinoma. High expression of PTN gene might be correlated with mechanism of multiple carcinoma. [ |
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| Keywords: | PTN gene cDNA microarray in situ hybridization gene expression regulation neoplastic neoplasms etiology |
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