慢性马兜铃酸肾病大鼠模型肾组织中蛋白质组的差异表达

目的:制备慢性马兜铃酸肾病的大鼠模型,建立马兜铃酸肾病大鼠肾组织和正常大鼠肾组织的双向电泳图谱,从而展示差异表达的蛋白质,为进一步筛选介导马兜铃酸毒性作用的蛋白质分子奠定基础. 方法:应用马兜铃酸腹腔注射制备大鼠慢性马兜铃酸肾病模型,提取其肾组织中总蛋白质,采用双向聚丙烯酰胺凝胶电泳技术,对模型组和正常组肾组织中蛋白质进行差异展示. 结果:成功制备了慢性马兜铃酸肾病大鼠模型,并建立了模型组和正常组大鼠肾组织的双向电泳图谱,发现了差异蛋白质. 结论:马兜铃酸导致大鼠肾组织中蛋白质差异表达,这些差异表达的蛋白质可能介导了马兜铃酸的毒性作用.

Differentially expressed proteins in renal tissues of rat models of chronic aristolochic acid nephropathy

AIM: To establish the 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE) profiles from aristolochic acid nephropathy (AAN) rat renal tissues and normal renal tissues and to identify the differentially expressed proteins. METHODS: Establish chronic renal interstitial fibrosis model in rats by peritoneal injection with aristolochic acid (AA). The total proteins from AAN models and normal rat renal tissues were separated by 2-D PAGE. The differentially expressed proteins in 2D-PAGE maps were analyzed using image analysis software. RESULTS: The rat models of AA-induced chronic renal interstitial fibrosis were established successfully. Clear, well-resolved and repro- ducible 2-D PAGE patterns of AAN models and normal rat renal tissues were obtained and many differentially expressed proteins were found. CONCLUSION: The differentially expressed proteins of renal tissues can be induced by AA in Wistar rats. These proteins maybe mediate the toxicity of aristolochic acid to renal tissues.