胶质瘤源性exosome的提取及形态学观察

目的证实体内和体外培养的胶质瘤细胞是否均可分泌exosome,为测定其蛋白质组成提供实验基础,为进一步利用exosome对胶质瘤进行免疫治疗提供理论依据。方法收集U251胶质瘤培养上清液和Ⅲ级星形胶质瘤囊液,利用差速离心法提取exosome,并用液相载网法在电镜下观察胶质瘤源性exosome的形态。结果 U251胶质瘤和Ⅲ级星形胶质瘤细胞均可以产生exosome,其平均直径约100 nm。结论证实了胶质瘤细胞像其他多数细胞一样,体内和体外培养均可分泌exosome。

Extraction and morphology observation of exosome from glioma cell

Objective To demonstrate whether the glioma cell can secrete exosome both in vivo and in vitro,to provide an experimental basis for the determination of the protein composition,to provide a theoretical basis for immunotherapy for the further use of exosome for glioma.Methods Extracting exosome from hydatid fluid of grade Ⅲ astroglioma cell and culture fluid of U251 glioma cell by differential centrifugation,observing the morphology of glioma cell derived exosome by electron microscope.Results All the U251 glioma cell and grade Ⅲ astroglioma cell can secrete exosome,the average diameter of the exosome is about 100 nm.Conclusion Confirmed that glioma cell,like most other cells,can secrete exosome both in vivo and in vitro.