海洛因诱导的条件性位置偏爱大鼠额叶联络皮层脑电的无线遥测及其分析

目的:利用条件性位置偏爱(CPP)视频系统结合脑电无线遥测技术,记录海洛因诱导的CPP大鼠额叶联络皮层(FrA)区的脑电变化,分析其与觅药行为之间的关系。方法:对大鼠FrA区进行脑立体定位电极埋藏,并分成对照组和海洛因诱导CPP组,后者制作海洛因依赖模型。利用无线遥测技术,分别记录2组大鼠黑白箱停留、黑-白穿梭和白-黑穿梭时大鼠FrA区脑电变化,分析其各波相对功率和百分比的差异。结果:海洛因诱导的CPP组大鼠黑白箱停留时遥测脑电各波相对功率和百分比,与对照组比较无显著差异(P>0.05),但海洛因诱导的CPP大鼠穿梭时左侧FrA脑电波呈现δ波减少、β波和β₂波增加的改变(P<0.05,P<0.01),尤以黑-白箱穿梭明显,而对照组大鼠穿梭时则表现出截然相反的脑电变化。结论:大鼠左侧FrA区慢波减少、快波增加的特异性改变,可能与海洛因诱导的CPP大鼠戒断状态下穿梭觅药行为及其动机形成有关。

EEG changes of front association cortex in heroin-induced CPP rats detected by wireless telemetry

AIM:To analyze the relationship between the electroencephalogram(EEG) changes of front association cortex(FrA) and the drug-seeking behavior by recording FrA EEG in heroin-induced conditioned place preference(CPP) rats. METHODS:A CPP video system combined with the technique of EEG wireless telemetry was used in the study. The rats were randomly divided into control group and heroin-induced CPP group after stereotaxic electrodes were buried on in the FrA, and the latter group was used as heroin dependence model. The FrA EEG was recorded by wireless telemetry,including staying in black or white chamber of video box, black-white chamber shuttle and white-black chamber shuttle. The differences of EEG were analyzed by the relative power and the percentage of each wave. RESULTS:When the rats stayed in the black or white chamber, no significant difference in the relative power and percentage of the telemetry EEG wave was observed(P>0.05). When the heroin-induced CPP rats shuttled between two chambers, especially shuttled between the black-white chambers, EEG on the left FrA showed that δ wave was reduced, and β wave together with β₂ wave was increased(P<0.05, P<0.01), while the EEG in control group showed the radically opposite changes. CONCLUSION:The specific changes of EEG on the left FrA including reduction in slow wave and increase in fast wave may be related to drug-seeking behavior and motivation in the rat withdrawal status.