胎鼠间充质干细胞移植缓解小鼠体力疲劳的研究

目的:观察胎鼠间充质干细胞(MSCs)缓解体力疲劳的作用,探讨其改善肌无力患者或者中老年体弱者生活质量的意义。方法:取孕13.5 d的BALB/c胎鼠,分离纯化得到胎鼠MSCs。6月龄BALB/c雌性小鼠随机分为安静对照组、单纯运动组和MSCs+运动组。MSCs+运动组尾静脉输注第5代MSCs, 其余2组输注同体积生理盐水。24 h后进行负重游泳试验,测定血尿素氮(BUN)、血乳酸、肝糖原和肌糖原,以及肝、肌肉、脑组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,心脏超声检查评价心功能。结果:(1)原代细胞呈梭形、多角形,散在、集落式分布;第5代细胞均匀平行排列或者漩涡状排列,表达CD44和CD105,不表达CD34和CD45,能向成骨细胞或脂肪细胞分化,具备MSCs基本特点。(2)MSCs缓解体力疲劳作用:MSCs+运动组与单纯运动组比较,负重游泳时间明显延长,BUN和血乳酸水平降低,肝糖原和肌糖原储备增加,肝脏和肌肉组织SOD活性升高,MDA水平降低,以上差异均显著(P＜0.05)。(3)心功能增强:MSCs+运动组与单纯运动组比较,每搏输出量、心输出量和左室舒张期容积均增加。结论:胎鼠间充质干细胞移植具有缓解小鼠体力疲劳作用。

Transplantation of mouse fetus-derived MSCs alleviates physical fatigue in mouse

AIM:To observe the effect of mouse fetus-derived mesenchymal stem cell(MSC) transplantation on alleviating fatigue in mice. METHODS:MSCs were derived from the mouse fetus at 13.5 d. The 6-month-old BALB/c mice were randomly divided into non-swimming group, swimming control group and swimming+MSCs group. The mice in swimming+MSCs group were injected with fetus-derived MSCs through the tail vein, while the mice in non-swimming group and swimming control group were injected with equal volume of normal saline. The anti-fatigue activity was evaluated using a weight-loaded swimming test, along with the determination of blood urea nitrogen(BUN), blood lactic acid(BLA),and glycogen in the hepatic and muscular tissues 24 h later. The antioxidant activity was evaluated by determining the activity of superoxide dismutase(SOD) and the content of malondialdehyde(MDA). The cardiac functions were measured by echocardiography. RESULTS:Primary cultured cells were spindle-shaped in scattered colony. The cells in fifth passage grew parallelly or swirlingly. The cells expressed CD44 and CD105, but not CD34 or CD45. Inducing experiments showed that the cells differentiated into osteoblasts or adipocytes. Transplantation of MSCs alleviated fatigue in mice. In swimming+MSCs group, weight-loaded swimming time was longer, the levels of BUN, BLA, hepatic and muscular MDA were lower, and the levels of glycogen and SOD in the hepatic and muscular tissues were higher than those in control group. Stroke volume, cardiac output and left ventricular diastolic volume in swimming+MSCs group increased, indicating that the cardiac functions were enhanced. CONCLUSION: Transplantation of mouse fetus-derived MSCs alleviates physical fatigue.