| 生长抑素对人球后成纤维细胞增殖的影响 |
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| 摘 要： |
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| 关 键 词： | Graves眼病 成纤维细胞 MTT比色法 生长抑素14 |
The influence of somatostatin 14 on the proliferation of human retroocular fibroblasts] |
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| Z An,S Wei,S Xu,H Yang. The influence of somatostatin 14 on the proliferation of human retroocular fibroblasts][J]. Journal of West China University of Medical Sciences, 2001, 32(3): 399-401 |
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| Authors: | Z An S Wei S Xu H Yang |
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| Affiliation: | Division of Endorcrinology, First Affiliated Hospital, WCUMS, Chengdu 610041, China. |
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| Abstract: | OBJECTIVE: To elucidate the possible mechanism of Somatostatin 14 in the treatment of Graves' ophthalmopathy, thus providing laboratory data with the intent to underpin therapeutic developments. METHODS: The human retro-ocular fibroblasts (HROFb) was cultured and MTT colorimetry assay was used to determine the proliferation of HROFb. RESULTS: MTT colorimetry assay could precisely reflect the proliferation of HROFb. The absorbance was directly proportional to the numbers of the cells (r = 0.996, P < 0.001). Somatostatin 14 could enhance the cell proliferation percentage at lower levels (< 25 nmol/L) and inhibit that at higher levels (> 25 nmol/L). The cell proliferation correlated positively to Somatostatin 14 levels significantly (r = -0.9054, P < 0.001). CONCLUSION: Somatostatin 14 has a dual effect on HROFb. |
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