Association between impaired IL-10 production following exposure to Staphylococcus aureus enterotoxin B and disease severity in eosinophilic chronic rhinosinusitis |
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Authors: | Takenori Haruna Shin Kariya Tazuko Fujiwara Takaya Higaki Seiichiro Makihara Kengo Kanai Rumi Fujiwara Satoshi Iwasaki Yoshihiro Noguchi Kazunori Nishizaki Mitsuhiro Okano |
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Affiliation: | 1. Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;2. Department of Otorhinolaryngology, International University of Health and Welfare School of Medicine, Narita, Japan;3. Department of Otorhinolaryngology, International University of Health and Welfare Mita Hospital, Tokyo, Japan;4. Department of Otorhinolaryngology, Kagawa Rosai Hospital, Marugame, Japan;5. Department Otorhinolaryngology, Kagawa Prefectural Central Hospital, Takamatsu, Japan;6. Department Otorhinolaryngology, International University of Health and Welfare Hospital, Nasushiobara, Japan |
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Abstract: | BackgroundIL-10 is a major anti-inflammatory cytokine that prevents inflammation-mediated tissue damage. We characterized the production of IL-10 by sinonasal tissue cells following exposure to Staphylococcus aureus enterotoxin B (SEB), which elicits cellular responses and is associated with the pathogenesis of eosinophilic chronic rhinosinusitis (ECRS).MethodsDispersed nasal polyp (NP) cells and uncinate tissue (UT) cells were prepared from patients with CRS with and without NP, respectively. Cells were incubated with SEB, and then the levels of IL-10 in the cell supernatants were determined. The effect of neutralizing IL-10 on SEB-induced IL-5, IL-13, IFN-γ, and IL-17A production was examined. Expression of IL-10 in NPs was also determined.ResultsIL-10 was expressed in infiltrating inflammatory cells in NPs. NP cells, especially non-adherent NP cells, produced substantial amounts of IL-10 in response to SEB. Although baseline production of IL-10 was significantly higher in NP cells than UT cells, the degree of IL-10 response to SEB was not significantly different between the cell types. The degree of IL-10 production was negatively correlated with the degree of eosinophilia both in tissues and peripheral blood whereas positively correlated with the 1-s forced expiratory volume/forced vital capacity ratio. Patients with severe ECRS displayed a significant decrease in IL-10 production compared with those with non-ECRS. IL-10 neutralization significantly augmented SEB-induced IL-13 and IFN-γ production by NP cells.ConclusionsImpaired IL-10 production in response to SEB in NP may exacerbate the pathophysiology of ECRS including eosinophilia and lower airway obstruction. |
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Keywords: | Eosinophilic chronic rhinosinusitis IL-10 JESREC criterion Nasal polyps Staphylococcal enterotoxin B CRS chronic rhinosinusitis CRSsNP chronic rhinosinusitis without nasal polyps CRSwNP chronic rhinosinusitis with nasal polyps ECRS eosinophilic chronic rhinosinusitis 1-s forced expiratory volume/forced vital capacity JESREC Japanese Epidemiological Survey of Refractory Eosinophilic Chronic Rhinosinusitis NP nasal polyps SEB staphylococcal enterotoxin B UT uncinate tissues |
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