| 胎儿骨髓间充质干细胞的分离培养及表面标志的检测 |
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| 引用本文： | 张燕,刘杰,王德文,崔雪梅,谷庆阳,董波,赵梅兰. 胎儿骨髓间充质干细胞的分离培养及表面标志的检测[J]. 细胞与分子免疫学杂志, 2002, 18(6): 631-633 |
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| 作者姓名： | 张燕 刘杰 王德文 崔雪梅 谷庆阳 董波 赵梅兰 |
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| 作者单位： | 1. 广州第一军医大学南方医院血液科实验室,广东,广州,510515
2. 军事医学科学院放射医学研究所实验病理学研究室,北京,100850 |
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| 摘 要： | 目的 建立胎儿骨髓间充质干细胞（mesenchymal stem cells,MSC)的分离培养方法，并对其表面标志进行检测。方法 采用体外细胞培养技术，分离培养胎儿骨髓MSC，用流式细胞术对其进行鉴定，并研究其增殖及生长特征。结果 流式细胞术证明，MSC具有间质细胞的特征。原代及传代培养显示，胎儿骨髓MSC具有活跃增殖的能力。结论 胎儿骨髓MSC作为组织工程重建的种子细胞具有较强的可行性。
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| 关 键 词： | 骨髓间充质干细胞 细胞培养 组织工程 |
| 文章编号： | 1007-8738(2002)06-631-03 |
The isolation, cultivation and surface marker detection of fetal bone marrow-derived mesenchymal stem cells |
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| ZHANG Yan ,LIU Jie ,WANG De wen ,CUI Xue mei ,GU Qing yang ,DONG Bo ,ZHAO Mei lan Laboratory of Hematology,Nanfang Hospital,First Military Medical University,Guangzhou ,Guangdong Province. The isolation, cultivation and surface marker detection of fetal bone marrow-derived mesenchymal stem cells[J]. Chinese journal of cellular and molecular immunology, 2002, 18(6): 631-633 |
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| Authors: | ZHANG Yan LIU Jie WANG De wen CUI Xue mei GU Qing yang DONG Bo ZHAO Mei lan Laboratory of Hematology Nanfang Hospital First Military Medical University Guangzhou Guangdong Province |
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| Affiliation: | ZHANG Yan 1,LIU Jie 2,WANG De wen 2,CUI Xue mei 2,GU Qing yang 2,DONG Bo 2,ZHAO Mei lan 2 1 Laboratory of Hematology,Nanfang Hospital,First Military Medical University,Guangzhou 510515,Guangdong Province,2 Department of Experimental Pathology,Institute of Radiation Medicine,Academy of Military Medicine Scienses,Beijing 100850,China |
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| Abstract: | Aim To establish a method for isolation and cultivation of fetal bone marrow derived mesenchymal stem cells (MSC) and to detect their surface makers. Methods MSC were isolated from bone marrow blood and purified via centrifuge and in vitro culture, MSC were analyzed by the flow cytometry. The proliferation and growth characteristics of MSC were observed during primary and passage cultures. Results Flow cytometry analysis showed that fetal bone marrow derived MSC exhibited the characteristics of mesenchymal cells, MSC had an active proliferative ability in primary and passage cultures. Conclusion Fetal bone marrow derived MSC has a great feasibility as the seed cells for tissue engineering reconstruction. |
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| Keywords: | mesenchymal stem cells cell culture tissue engineering |
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