胎盘来源间充质干细胞的体外诱导成软骨分化

背景：胎盘间充质干细胞已被证实有较强的增殖能力,且能向成骨细胞、成神经细胞、类肝样细胞等诱导分化,但对其成软骨细胞诱导分化的研究不多。目的：在体外诱导人胎盘间充质干细胞成软骨细胞分化。方法：体外培养人胎盘间充质干细胞并鉴定。取第3代胎盘间充质干细胞,调整细胞悬液浓度为1.6×1010 L-1,在24孔板中央分别滴5,10,15μL细胞悬液,培养2 h（目的是形成微团）;然后加入间充质干细胞培养基或软骨诱导培养基培养14 d,阿利新蓝染色,进行大体观察及倒置显微镜观察。结果与结论：应用间充质干细胞培养基培养的对照组,细胞增殖形成大量贴壁细胞,贴壁细胞具有典型的间充质细胞形态;应用软骨诱导培养基培养后,细胞只保持微团,不继续增殖形成贴壁细胞,微团基部无贴壁细胞,随着接种细胞数量的增加,微团直径增大。说明人胎盘来源间充质干细胞具有向软骨细胞分化的能力,可作为组织工程、细胞治疗等应用的种子细胞来源。

Chondrogenic differentiation of placenta-derived mesenchymal stem cells in vitro

BACKGROUND： Placenta-derived mesenchymal stem cells have been shown to have a strong proliferative capacity, and can differentiate into osteoblasts, neuroblasts, hepatocyte-like cells, but there are few studies about their chondrogenic differentiation. OBJECTIVE： To induce the differentiation of human placenta-derived mesenchymal stem cells into chondrocytes in vitro. METHODS： Human placenta-derived mesenchymal stem cells were culture in vitro and identified. Passage 3 cells were used to prepare cell suspensions at the concentration of 1.6×1010 cells/L, and then cultured in 24-well plates at a volume of 5, 10, 15 μL for 2 hours for the purpose of forming micelles. After that, the samples were cultured in mesenchymal stem cell medium or cartilage induction medium for 14 days. Alcian blue staining, general observation and inverted microscope observation were performed. RESULTS AND CONCLUSION： A great amount of adherent cells formed in the mesenchymal stem cell medium, which possessed typical morphology of mesenchymal stem cells. After cultured in cartilage induction medium, the cells only maintained micelles and did not proliferate continuously to form adherent cells. The base of these micelles had no adherent cells, and the diameter of micelles became increasing with the increasing number ofinoculated cells. These findings suggest that human placenta-derived mesenchymal stem cells have the chondrogenic ability, and can be used as seed cells for tissue engineering and cell therapy.