救脑宁注射液对培养神经细胞缺氧缺糖损伤的保护作用

为了探索“救脑宁”注射液对中风病的疗效机理 ,将原代培养 8～ 12d的新生大鼠皮层神经细胞进行缺氧缺糖处理 ,观察该药对缺氧缺糖损伤神经细胞的影响。结果表明 :缺氧缺糖组细胞上清液中丙二醛 (MDA)含量、乳酸脱氢酶 (LDH)活性较对照组显著升高 ,细胞超氧化物岐化酶 (SOD)活性和细胞生存率则显著降低 ,救脑宁组MDA、LDH显著低于缺氧缺糖组 ,而SOD活性及细胞生存率则高于缺氧缺糖组 (P <0 0 1)。因此 ,抗脂质过氧化损伤、提高神经细胞对缺氧缺糖的耐受性 ,可能是其疗效机理之一。

Protective Effects of Jiunaoning Injection against the Lesions of Cultured Nerve Cells Caused by Oxygen and Glucose Deprivation

The cortical cells of the new-born rat cultured for 8～12 days by the primary culture method were treated by oxygen and glucose deprivation (OGD), an d the effects of Jiunaoning Injection (JI) on the OGD-injured cortical cells we r e observed. The results showed that the MDA level and the LDH activity in the ce ll supernate were significantly increased, while the SOD activity and cell survi val rate were significantly decreased in the OGD group, compared with those in t h e control group; and the MDA level and the LDH activity were significantly decre ased, while the SOD activity and cell survival rate were increased in the JI gro up, compared with those in the OGD group. The results suggest that the basis of t he clinical effects of JI is relieving the lesions caused by lipid peroxidation and increasing the tolerance of nerve cells to OGD.