硒对实验性大鼠肝纤维化的影响

目的:观察不同剂量补硒对大鼠血清硒含量及实验性肝纤维化的影响。方法:将Wistar大鼠50只分为正常对照组、四氯化碳(CCl4)模型组、高糖组、补硒高剂量组和低剂量组,后4组动物每只给CCl4油溶液0.15ml腹腔内注射2次/周,共注射6周;后3组大鼠分别给予高糖、葡萄糖酸硒,观察每一种情况对大鼠血清硒含量、肝功能和肝纤维化形成过程产生的影响。结果:实验3周和6周时,补硒组和模型组及高糖组血清ALT均增高,各组间差异不明显,但补硒组AST均明显降低,与模型组及高糖组比较有较明显差异(P<0.05或P<0.01),补硒组大鼠血清硒含量在6周时均较模型组明显增高(P<0.01),维持在正常水平,并对大鼠肝纤维化有一定的抑制作用,但两个剂量组间未见明显差异,高糖使大鼠的体重明显增加,未见大鼠肝纤维化的改善。结论:补硒对肝损伤动物血清硒及肝纤维化的影响表现在后期,采用低剂量较长期的补硒方法可能有效安全。

The Effect of Selenium on the Hepatic Fibrosis of Rats

Objective:To observe the effect of supplying different level of selenium on the content of selenium in rat serum and experimental hepatic fibrosis.Methods: 50 wistar rats were divided into the healthy control group,model CCl_4 group,high content of sugar group,high dosage of selenium group and low dosage of selenium group,and the last four groups were injected with 0.15 ml CCl_4 oily liquor two times per week,totally 6 weeks.The last three groups were respectively administered sugar in high content,glucose acid selenium in order to observe the effect of each case on the content of selenium in rat serum,the function of liver and the course of hepatic fibrosis.Results: The serum ALT increased at the third week and the sixth week in using selenium group,model CCl_4 group and high content of sugar group,but there was obvious difference in the content of AST between the model group,high sugar group and the selenium group which the content of AST was distinctly decreased(P<0.05 or P<0.01),the content of serum selenium in the two selenium groups was all obviously higher than the model group(P<0.01) at the sixth week,maintaining at the normal level and having the negative effect on the hepatic fibrosis of rats.There was no obvious difference between the two selenium groups.The high content of sugar caused increase in the weight of rats and the hepatic fibrosis did not decreased in severity in the high content of sugar group.Conclusion: The effect of supplying selenium on the serum selenium of animals with liver injury and the hepatic fibrosis represents at the late time,and long term and low content of supplying selenium may be more effective and safe.