| 核磁共振技术快速检测单纯疱疹病毒Ⅱ型感染细胞的实验研究 |
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| 引用本文： | 刘剑雄,李晓华,袁汉珍.核磁共振技术快速检测单纯疱疹病毒Ⅱ型感染细胞的实验研究[J].武汉大学学报(医学版),1992(2). |
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| 作者姓名： | 刘剑雄 李晓华 袁汉珍 |
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| 作者单位： | 湖北医学院附一医院检验科，湖北医学院附一医院检验科，湖北医学院中国科学院武汉分院波谱及原子分子实验室 |
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| 摘 要： | 选用核磁共振技术进行病毒学诊断实验研究结果表明:单纯疱疹病毒Ⅱ型感染细胞后1 h,随着病毒悬液稀释度的不同(10~9～10~(-3)),细胞悬液的自旋晶-格驰豫时间 T_、从11.847 s 相应地缩短至7.807 s,当病毒悬液稀释度为10~(-4)时,细胞悬液的 T_1为12.388 s,恢复到正常对照细胞悬液的 T_1水平(12.175 s)。用不同滴度的抗 HSV-Ⅱ血清中和 HSV-Ⅱ,细胞悬液 T_1相应地逐步恢复至正常。实验研究证实了病毒感染细胞后1 h 就能引起细胞溶液自旋-晶格驰豫时间的特征性改变.运用核磁共振技术可望为临床快速诊断病毒感染提供一条新型、可靠的途径。
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| 关 键 词： | 疱疹病毒 病毒 感染 核磁共振/方法 |
Experimental Study on Detection Virus-infectes Cell with Nuclear Magnetic Resonance Technique |
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| Liu Jianxiong Li Xiaohua.Experimental Study on Detection Virus-infectes Cell with Nuclear Magnetic Resonance Technique[J].Medical Journal of Wuhan University,1992(2). |
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| Authors: | Liu Jianxiong Li Xiaohua |
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| Abstract: | We make the study on virelogical diagnosis with nuclear magnetic resonance(NMR)technique. The results obtained showed that the Hep-2 cell water proton spinlattice relaxation time(T_1)changed from 7+807 s to 11.847 s in cell cultures with variance dilution of infection(t0~(-3)～10~0)after 1 h adsorption with HSV-Ⅱ preparations at 37℃,the cell control water T_1 was 12.175s,After 1 h ad- sorption with 10~(-4)HSV-Ⅱ preparation at 37℃,the cell Water T_1 was 12.388 s and returned to the level of the cell control water T_1.When HSV-Ⅱ was neutralized by specific anti-HSV-Ⅱ antisorum, it could prevent the change in cell water T_1.The result was similar to the cytopathic effect(CPE).It is confirmed that occured a characteristic change of the cell water T_1 in cell culture after 1 h virus in- fection.These experiments demonstrate that NMR technique is a reproducible,specific,rapidly and easlly method for detection of virusinfceted cell. |
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| Keywords: | MeSH herpesviruses viruses infection nuclear magnetic resonance/MT |
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