淋巴管内皮细胞与PGA的相容性研究

目的研究人淋巴管内皮细胞(Lymphatic endothelial cells,LEC)与可吸收生物材料聚羟基乙酸(Polyglycolic acids,PGA)的相容性,为组织工程化淋巴管的构建奠定基础。方法采用免疫磁珠分离法,从幼儿包皮组织中分离出LEC,扩增至第3代,与PGA混合培养,形成细胞-生物材料复合物。每两天换液1次,观察细胞形态和黏附生长状况。体外培养1周后,行电镜观察和RT-PCR检测。结果复合物体外培养1周后,LEC黏附在PGA支架上,细胞分泌大量基质。RT-PCR提示Podoplanin、LYVE-1、VEGFR-3和Prox-1均呈阳性表达,LEC表型未发生改变。结论 LEC与PGA有良好的细胞相容性,PGA可作为组织工程化淋巴管构建的支架材料。

Experimental Research on Cell Biocompatibility of Polyglycolic Acids with Lymphatic Endothelial Cells

Objective To investigate the cell biocompatibility of Polyglycolic acids （PGA） with lymphatic endothelial cells （LEC） in vitro. Methods LECs were isolated from human neonatal foreskin dermis by MACS and multiplied in culture. LECs of the third passage were collected and then seeded onto PGA unwoven fibers. The cell-biomaterials complex were cul- tured in EGM-2-MV, and the medium was changed every 2 days. At the first week, small pieces of the LEC-PGA sheets were cut off for scanning electron microscope examination and RT-PCR analysis. Results At the first week, LECs adhered the PGA scaffolds and secreted much of extracellular matrix proteins. RT-PCR revealed the expression of Podoplanin, LYVE-1, VEGFR-3 and Prox-1 in gene. Conclusion PGA presented good biocompatibility with LECs and could be used as lymphatic tissue engineering scaffold.