| RP-HPLC测定茅苍术中β-桉叶醇的含量 |
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| 引用本文： | 陈炎明,侴桂新,王峥涛.RP-HPLC测定茅苍术中β-桉叶醇的含量[J].中国中药杂志,2007,32(21):2265-2267. |
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| 作者姓名： | 陈炎明 侴桂新 王峥涛 |
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| 作者单位： | 上海中医药大学中药研究所教育部中药标准化重点实验室，上海中医药大学中药研究所教育部中药标准化重点实验室，上海中医药大学中药研究所教育部中药标准化重点实验室 上海201203，上海中药标准化研究中心，上海201203，上海201203，上海中药标准化研究中心，上海201203，上海201203，上海中药标准化研究中心，上海201203 |
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| 基金项目： | 国家中医药管理局中医药科技重大项目专项项目(02代08)，上海市重点学科建设项目(Y0301)，上海市科委科研计划项目(03DZ19546) |
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| 摘 要： | 目的：采用RP-HPLC测定茅苍术中β-桉叶醇的含量,为茅苍术质量标准研究提供依据。方法：采用反相高效液相色谱法,色谱柱为Inertsil ODS-3(4.6 mm×250 mm,5 μm)；流动相乙腈-水(68∶32)；流速1.0 mL·min-1；检测波长200 nm；柱温为25 ℃。结果：测得10批茅苍术药材中β-桉叶醇含量为0.833～4.466 mg·g-1,β-桉叶醇为0.048～1.200 μg,r=0.999 9,平均回收率99.3%,RSD 1.4%(n=9)。结论：建立了高效液相色谱法测定茅苍术中β-桉叶醇含量的方法,该方法准确,可靠,可用于茅苍术药材的测定。
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| 关 键 词： | 茅苍术 β-桉叶醇 高效液相色谱法 含量测定 |
| 文章编号： | 1001-5302（2007）21-2265-03 |
| 收稿时间： | 2006-11-15 |
| 修稿时间： | 2006年11月15 |
Determination of beta-eudesmol in rhizome of Atractylodes lancea by RP-HPLC] |
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| CHEN Yan-ming; CHOU Gui-xin; WANG Zheng-tao.Determination of beta-eudesmol in rhizome of Atractylodes lancea by RP-HPLC][J].China Journal of Chinese Materia Medica,2007,32(21):2265-2267. |
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| Authors: | CHEN Yan-ming; CHOU Gui-xin; WANG Zheng-tao |
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| Institution: | Key Laboratory of Standardization of Chinese Medicine, Ministry of Education, Institutes of Chinese Material Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. |
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| Abstract: | OBJECTIVE: To develop a RP-HPLC method for determination of beta-eudesmol in rhizome of Atractylodes lancea, and to provide valuble data for quality control of A. lancea. METHOD: The samples were separated on an Inertsil ODS-3 (4.6 mm x 250 mm, 5 microm) column with the mobile phase of acetonitrile-water (68:32). Flow rate was 1.0 mL x min(-1). The detection wavelength was set at 200 nm. Column temperature was 25 degrees C. RESULT: The contents of beta-eudesmol determinated was 0.833-4.466 mg x g(-1), The linear range of beta-eudesmol was 0.048-1.200 microg (r = 0.999 9), the average recovery was 99.3%, RSD was 1.4% (n = 9). CONCLUSION: The method for quantitation of beta-eudesmol in A. lancea was accurate and reliable, which can be used to evaluate the quality of rhizome of A. lancea. |
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