逼尿肌反射亢进和逼尿肌无反射大鼠膀胱Cajal间质细胞数量变化

目的观察脊髓损伤后大鼠逼尿肌反射亢进和逼尿肌无反射膀胱Cajal间质细胞(ICC)数量变化,探讨膀胱逼尿肌功能改变和ICC的可能关系。方法40只大鼠随机分为正常组、膀胱造瘘组、逼尿肌反射亢进组和逼尿肌无反射组,每组10只。制作大鼠骶髓和骶髓上损伤模型,尿动力学检查证实膀胱出现逼尿肌反射亢进和逼尿肌无反射后,取膀胱组织行免疫组织化学染色,激光共聚焦显微镜观察ICC数量变化。结果各组膀胱ICC数量比较:正常组与膀胱造瘘组比较差异无统计学意义(P>0.05);逼尿肌反射亢进组明显高于正常组和膀胱造瘘组,差异有统计学意义(P<0.05);逼尿肌无反射组明显低于正常组和膀胱造瘘组,差异有统计学意义(P<0.05)。结论脊髓损伤后大鼠膀胱功能改变可能与ICC数量变化有关。

Quantitative change of interstitial cells of Cajal in rats bladder with detrusor hyperreflexia or detrusor areflexia

Objective To investigate the quantitative change of interstitial cells of Cajal （ICC） in rats bladder with detrusor hyperreflexia or detrusor areflexia after spinal cord injury and explore the relationship between the function changes of detrusor of bladder and ICC. Methods Forty rats were randomly divided into normal group, post-cystostomy bladder group, detrusor hyperreflexia bladder group and detrusor areflexia bladder group, ten rats in each group. The rats models with suprasacral cord injury and sacral cord injury were made, and urodynamic studies were conducted to confirm that bladder respectively expressed detrusor hyperreflexia and detrusor areflexia, then specimens of rats bladder were stained immunohistochemically with a c-kit antibody, and the number of ICC was observed with a laser confocal microscope. Results There was no statistic difference in the number of ICC between normal group and post-cystostomy group（ P 〉 0.05 ）. The number of ICC was significantly higher in detrusor hyperreflexia bladder group than that in normal and post-cystostomy bladder group （ P 〈 0.05 ）. The number of ICC was significantly lower in detrusor areflexia bladder group than that in normal and post-cystostomy bladder group （ P 〈 0.05 ）. Conclusion It is possible that the function changes of bladder after spinal cord injury may be related to changes of the number of ICC.