Antihyperglycemic effects of total flavonoids from Polygonatum odoratum in STZ and alloxan-induced diabetic rats |
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Authors: | Xiao-Shun Shu Jin-Hai Lv Jun Tao Guo-Ming Li Huai-Den Li Ning Ma |
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Institution: | 1. College of Biology & Chemistry Engineering, Changsha University of Science & Technology, Changsha 410076, PR China;2. Industry Institute Zhuzhou National-Level Hi-Tech and Industry Development Zone, Zhuzhou 410076, PR China;3. Department of Biology, Huaihua College, Huaihua 418000, PR China;4. The Clinical Pharmacy & Pharmacology Institute, Second Xiangya Hospital, Central South University, Changsha 410011, PR China;5. Changsha Medical College, Changsha 410219, PR China |
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Abstract: | Aim of the studyTotal flavonids of Polygonatum(P) odoratum (TFP) were tested for anti-diabetic activity in streptozotocin (STZ)-induced diabetic mice and alloxan-induced diabetic rats.Materials and methodsRhizoma Polygonati Odorati, well-known Chinese traditional medicine, is widely used for treatment of diverse diseases for example diabetes. In our study, TFP was extracted by 70% ethanol and purified by macroreticular resin. The experiments were designed to detect the anti-diabetic activity of TFP by determination of blood glucose (BG) using one touch gluco-meter and insulin levels by using a radioimmunoassay kit in streptozotocin (STZ)-induced diabetic mice and alloxan-induced diabetic rats and alpha-amylase inhibitory activity by alpha-amylase inhibition assay in vitro.ResultsTFP had beneficial effects on regulation of blood glucose. Daily administration with 50–200 mg/kg body weight of TFP for 9 days can reduce significantly hyperglycemia in STZ-induced diabetic mice. Thirtieth day administration with TFP (50–200 mg/kg body weight) also decreased significantly fasting blood glucose in alloxan-induced diabetic rats. The hypoglycemic effect of TFP at 50 and 100 mg/kg is less than that of acarbose 20 mg/kg and gliclazide 15 mg/kg. The hypoglycemic effects of TFP at 200 mg/kg is similar to that of acarbose 20 mg/kg and gliclazide 15 mg/kg. TFP also could increase significantly the insulin level in alloxan-induced type 2 diabetic rats (P < 0.05) compared with control. Alpha-amylase inhibition assay in vitro showed that TFP inhibited significantly alpha-amylase activity in a dose-dependent manner.ConclusionsTFP possess significant dose-dependent anti-diabetic activity. TFP is one of the primary hypoglycemic active compounds of Polygonatum odoratum which would worth further study and development. |
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Keywords: | Polygonatum odoratum Anti-diabetic plant Hypoglycemic effect Total flavonoids |
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