Active constituents from Sophora japonica exhibiting cellular tyrosinase inhibition in human epidermal melanocytes |
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Authors: | Yuan-Hsin Lo Rong-Dih Lin Yi-Pei Lin Yan-Ling Liu Mei-Hsien Lee |
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Institution: | 1. Department of Dermatology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei 111, Taiwan;2. Department of Internal Medicine, Ho-Ping Branch, Taipei City Hospital, Taipei 100, Taiwan;3. School of Bio-resources Technology, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan;4. School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan;5. Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan;6. Center for Reproductive Medicine & Sciences, Taipei Medical University Hospital, Taipei 110, Taiwan |
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Abstract: | Aim of the StudyThere is greater consumer awareness of plant-based skin-care products. Sophora japonica L. (Fabaceae) has been used traditionally as a hemostatic agent and also has skin-care and whitening benefits. The effect of the isolated active compounds of Sophora japonica L. (Fabaceae) that inhibits tyrosinase activity in human epidermal melanocytes (HEMn) was examined.Materials and methodsWe used the mushroom tyrosinase inhibitory assay to isolate active constituents from the extracts. The structures of these constituents were characterized by physical and spectroscopic analyses. Cellular tyrosinase kinetics were analyzed and showed by Lineweaver–Burk plot.ResultsA new compound, N-feruloyl-N′-cis-feruloyl-putrescine (8), together with four flavonoids and three putrescine derivatives were obtained after assay-guided isolation of S. japonica. In HEMn, compound 8 was minimally cytotoxic (cell viability >90% at 100 μM) and the IC50 value for suppression of cellular tyrosinase activity was estimated as 85.0 μM. Zymography analysis demonstrated the compound's concentration-dependent effects and the kinetic analysis indicated the compound's mixed-inhibitory action.ConclusionsWe concluded that the new compound 8 is the most potent component of S. japonica yet discovered. Its pigment inhibition activity may be exploitable cosmetically. |
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Keywords: | Sophora japonica Fabaceae N-Feruloyl-N&prime -cis-feruloyl-putrescine Human epidermal melanocytes Tyrosinase |
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